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Polymorphism of Porcine Uncoupling Protein 3 (UCP3) and Its Association
with Fat Deposition and Meat Quality |
| REN Liang;ZHU Bao-qin;HAN Dan;ZHANG Yi-bo;SONG Hui-juan;ZENG Rui-xia;SU Yu-hong |
| Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, Jinzhou Medical College, Jinzhou 121000, China |
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Abstract Based on quantitative traits loci (QTL) mapping, porcine uncoupling protein 3 (UCP3) gene was chosen as a candidate gene for pig fat deposition and meat quality traits. Partial coding region of UCP3 gene was sequenced and there was one single nucleotide polymorphisms (cSNP) at 395 bp. This mutation was G→A and it resulted in the amino acid change from glycine to arginine. This site was also recognized by restriction endonuclease SmaⅠ. The polymorphism of the UCP3 SmaⅠwas analyzed with PCR-RFLP among 186 Large white × Meishan F2 progeny. The genotypes of UCP3 SmaⅠwere AA, AB and BB. The frequency of A allele was 0.56 and B was 0.44. With statistical analysis, it was found that the SmaⅠ polymorphism in the F2 population was significant associated with backfat thickness at thorax-waist and at buttock, intramuscular fat, drip loss rate and water holding capacity. The function of UCP3 SmaⅠmainly showed additive effect. The genotype AA reduced back-fat thickness and water drip loss rate, and increased water holding capacity. Meanwhile, it also decreased the intramuscular fat content .
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Received: 25 July 2005
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Corresponding Authors:
SU Yu-hong
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