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Transgenic Plants of Brassica campestris with Resveratrol Synthase Gene |
HU Xiao-ping;SHANG Wen-jing;CAI Wen-qi;HAN Qing-mei;KANG Zhen-sheng |
College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, China |
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Abstract A plant constitute expression vector EHA105/pBinRS, which contained NPTⅡ and resveratrol synthase (RS) gene, was constructed through plasmid pBin438. RS gene was then transferred into Brassica campestris ssp. chinensis using an Agrobacteriume tume faciens mediated method. The regeneration plants were screened on MSBk medium and assayed by PCR and RT-PCR. The results showed that RS gene had been integrated into genome of B. campestris ssp. chinensis and normally expressed. And seven transgenic plants were obtained.
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Received: 21 February 2005
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Corresponding Authors:
KANG Zhen-sheng
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