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Tissue Specificity of Somatostatin(SS) mRNA Expression in Gastrointestinal Tracts of Chicken |
WANG Xiu-qi;TAN Hui-ze;SU Hai-lin;DAI Fa-wen;FENG Ding-yuan |
College of Animal Science, South China Agricultural University,Guangzhou 510642, China |
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Abstract The objective of this study is to investigate the the tissue specificity of somatostatin (SS ) mRNA expression in gastrointestinal tracts by relative quantitative RT-PCR . Arbor Acre(AA) broilers at age of 30 days were used and the gastrointestinal tracts were sampled. The result showed that SS mRNA expression in glandular stomach was higher than that in intestine segment(P<0.01);Duodenum and jejunum expressed SS mRNA similarly and their mRNA abundance was higher than that of ileum(P<0.05); Qualitative analysis showed that muscular stomach and colorectum could express SS mRNA, but the abundance was significantly lower than that of glandular stomach, duodenum, jejuneum and ileum. The abundant expression of SS mRNA in digestion organ (glandular stomach) and absorption organ (duodenum and proximal jejunum) indicate that SS has important function on regulating the digestion and absorption of nutrients, but the mechanism needs further research.
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Received: 26 April 2005
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Corresponding Authors:
FENG Ding-yuan
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