|
|
|
| Construction of Antagonistical, Insecticidal and Endophytic Multifunctional-engineering Strains by Protoplast Fusion |
| YAN Nian-Long1 QIU Si-Xin2 HE Hong3 GUAN Xiong1 HU Fang-Ping2** |
(1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. Agricultural College, Zhanjiang Ocean University, Zhanjiang 324088, China) |
|
|
|
|
Abstract Abstract： A multifunctional-engineering strain was constructed by protoplast fusing of the insecticidal Bacillus thuringiensis with the endophytic B. subtilis. The results of PCR, insecticidal and endophytic tests showed that the vip3A gene was existed in the fusant, the antagonism of the fusant was against the Fusarium oxysporum f.sp.cucumerinum and the adjusted mortality to the Plutella xylostella reached 59%, at the same time, its endophytic character was also existed.
|
|
Received: 01 January 1900
|
|
Corresponding Authors:
HU Fang-Ping
|
|
|
|
|
|
|
