Abstract Abstract: Using phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) as indicators of grape resistance to pathogen infection, the effects of different components (bacterial cells, fermentation products and metabolites) of strain E26(Agrobacterium vitis ), biocontrol agent of crown gall, on PAL activities and genes' transcriptions of pal and sts in suspension cells of two grape varieties Vitis romanetii cv. Qiuhe and V. vinifera cv. Cabernet Sauvignon) were studied. The PAL activities of Qiuhei and Cabernet Sauvignon were higher than the controls at 12 h to 48 h after treated by E26 bacterial cells, 4 h to 48 h after treated by E26 fermentation products and 12 h to 48 h after treated by E26 metabolites. Dot blotting analysis showed that the quantities of pal and sts transcriptional products (mRNA) both in Qiuhei and Cabernet Sauvignon were higher than that of the control at 12 h to 48 h after treated by E26 bacterial cells; At 4 h to 48 h after treated by E26 fermentation products, the quantities of pal transcriptional products in Qiuhei were higher than that of the control, while that in Cabernet Sauvignon were 4 h to 12 h. The quantities of sts transcriptional products in the two varieties were higher than that of control at 4 h to 48 h after treated by E26 fermentation products; At 4 h to 48 h after treated by E26 metabolites, the quantities of pal transcriptional products in Qiuhei were higher than that of the control, while that in Cabernet Sauvignon were 4 h to 24 h. The quantities of sts transcriptional products in the two varieties were higher than that of the control at 6 h to 48 h after treated by E26 metabolites.
ZHENG Zu-Yu LI Jin-Yun WANG Jian-Hui WANG Hui-Min**. Effects of E26 Agent on the Expressions of Defense Genes pal and sts of Grape Suspension Cells[J]. , 2004, 12(6): 698-703.
