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| S1 Nuclease Sensitivity of Surrounded Nucleolus(SN)- and Non Surrounded Nucleolus(NSN)-type Mouse Ooctyes during In vitro Maturation |
| LI Jun YANG Ming-Sheng LI Ying-Xiang XU Yin-Xue LIU Hong-Lin** |
| (College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) |
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Abstract Abstract: The fully grown mouse germinal vesicle oocytes from large antral follicle can be classified into two groups, according to their chromatin configuration. The S1 nuclease sensitivity of them was investigated during their maturation in vitro. The results showed that NSN-type (non-surrounded nucleolus, whose decondensed chromatin distributed in almost whole nuclear) oocytes at MⅠ stage had more S1 nuclease sensitivity than that of SN-type (surrounded nucleolus，whose condensed chromatin surrounded nucleolus.) oocytes (P <0.01), while little difference between them in S1 nuclease sensitivity was found at MⅡ stage (P >0.05). It seemed that the NSN-type oocytes at MⅠ stage had more abundant single-strand property than SN-type oocytes, and during developing from MⅠ stage to MⅡ stage, the abundance of single-strand character in these two-type oocytes became homogenized.
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Received: 01 January 1900
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Corresponding Authors:
LIU Hong-Lin
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