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Structural Analyses of Functional Region of Maize
In5-2 Promoter in Transgenic Tobacco |
YUAN Yuan LIANG Ben-Guo LIU Yun-Jun WANG Tao** |
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Abstract Abstract: The different length of six maize In5-2 promoter 5' end deletions were obtained by PCR method. The mutants were cloned to plant expression vector D1301 and transferred into Nicotiana tabacum. GUS activity analysis showed that regulation region of its basic transcription was mainly within -1 520 to -1 431 bp upstream of ATG. DNAMAN analysis there were two stem-loop structures within -1 108 to -1 079 and –891 to -864 bp related to ATG. It was suggested that their protein integration site was between -1 079 to -897 bp related to ATG. In addition, cis-acting elements responsive to chloroacetanilide could be mainly located within the range of-388 to -86 bp upstream of ATG.
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Received: 01 January 1900
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Corresponding Authors:
WANG Tao
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