Development and Preliminary Application of the gE Enzyme Linked Immunosorbent Assay for Detecting Antibodies to gE Protein of Pseudorabies Virus in Pigs
TANG Yong1,2 CHEN Huan-Chun1** QIN Ya-Li1 HE Qi-Gai1 JIN Mei-Li1 WU Bin1 LIU Zheng-Fei1
(1. Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 2. Department of Bioengineering, Jinan University, Guangzhou 510632, China)
Abstract Abstract: To differentiate pigs infected with pseudorabies virus (PrV) and vaccinated with Ge-PrV, the gE enzyme linked immunosorbent assay(ELISA) based on the recombinant glycoprotein E, which was expressed by E .coli, purified, denatured and renatured, was developed. The diagnostic specificity and sensitivity of the developed gE-ELISA were evaluated by testing 115 serum samples. The gE-ELISA's diagnostic specificity was 94.5% and sensitivity was 96.7%. Five serum samples were tested with plates from 5 patches and a coefficient of variation of less than 10% was obtained, showing the gE-ELISA had good reproducibility. When gE-ELISA was compared with a commercial blocking ELISA by testing 356 serum samples, the agreement rate of the two assays was 92.13%(328/356). The above results indicated that the gE-ELISA developed in our laboratory could be used to differentiate the PrV infected and gE-PrV vaccinated pigs.
TANG Yong, CHEN Huan-Chun,** QIN Ya-Li, et al. Development and Preliminary Application of the gE Enzyme Linked Immunosorbent Assay for Detecting Antibodies to gE Protein of Pseudorabies Virus in Pigs[J]. , 2004, 12(6): 635-638.
