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β-glucosidase cDNA Cloning in the Tea(Camellia sinensis )
and Its Prokaryotic Expression
 LI Yuan-Hua JIANG Chang-Jun** YANG Shun-Li YU You-Ben
(Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Agriculture,
Anhui Agricultural University, Hefei 230036, China)
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Abstract  Abstract:The β-glucosidase gene has important effects on the alcoholic aroma precursors and insect-resistance. The complete cDNA squence of β-glucosidase of tea(Camellia sinensis) was cloned  and its full length was 1 475 bp (GenBank, Accession No. AF537127), and shared 40%~60% similarity to corresponding parts of β-glucosidase gene from other plants in nucleotide sequence. Its secondary structure contained 14.33% α-helical conformation, 25.43% β-sheet conformation and many function domains of amino acid. The β-glucosidase gene was cloned into the pET-32a expression vector and expressed high-efficiently in Escherichia coli  BL21(DE3), and the molecular weight of expressed fusion protein was 63 kD. The results of emzymatic reaction showed that fusion protein possesed normal bioactivity, and it could catalyze the dehydration of the glycodic bond. The fusion protein was mainly expressed  by soluble protein in cytoplasm.
Key wordsKey words: tea(Camellia sinensis )      β-glucosidase      cDNA      prokaryotic expression      
Received: 01 January 1900     
Corresponding Authors: JIANG Chang-Jun   
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 LI Yuan-Hua JIANG Chang-Jun** YANG Shun-Li YU You-Ben
Cite this article:   
 LI Yuan-Hua JIANG Chang-Jun** YANG Shun-Li YU You-Ben. β-glucosidase cDNA Cloning in the Tea(Camellia sinensis )
and Its Prokaryotic Expression
[J]. , 2004, 12(6): 625-629.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2004/V12/I6/625
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