Relationship of Lignin Content with the Stem Color in the Transgenic Poplar with Depressed Expression of 4CL Gene
Relationship of Lignin Content with the Stem Color in the Transgenic Poplar with Depressed Expression of 4CL Gene
(1.Beijing Agro-biotechnology Research Center, Beijing 100089, China; 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3. College of Forestry, Hebei Agriculture University, Baoding 071000, China)
Abstract Abstract:The lignin content of transgenic triploid Chinese white poplar (Populus tomentosa Carr.) expressing the antisense 4CL (4-coumarate:CoA ligase) cDNA was remarkably reduced. The stems of transgenic poplars were complete red-brown or mottled red-brown immediately after the bark was peeled off, while the stems of the untransformed plants were white. The reductions in lignin content of transgenic plants with complete red-brown stems were much higher than with mottled red-brown ones. Wiesner reactions of untransformed plants were typical purple-red, while that of complete red-brown stems of transgenic plants exhibited red. Wiesner reactions of white parts in mottled red stems of transgenic plants displayed typical purple red like untransformed plants, while that of red-brown parts showed red color, which testified further the relationship of lignin content with stem color in transgenic plant. It was considered that the stem color of transgenic plant could be employed as supplementary trait to screen the transgenic plant with the reduced lignin content.
Relationship of Lignin Content with the Stem Color in theTransgenic Poplar with Depressed Expression of 4CL Gene. Relationship of Lignin Content with the Stem Color in the Transgenic Poplar with Depressed Expression of 4CL Gene[J]. , 2004, 12(6): 621-624.
