Abstract The Zhijin white goose (Anser cygnoides orientalis) is a local breed developed through long-term artificial selection and terroir domestication, and it possesses the traits of tough feeding resistance and great adaptability. To investigate the accurate genetic information and to screen the loci associated with body size traits in Zhijin white goose, in this study, microsatellite technology was used to analyze the genetic diversity within the Zhijin white goose population and performed multiple comparisons of different genotypes in loci related to body size, aiming to provide a theoretical basis for the conservation and development of germplasm resources of Zhijin white goose. A total of 101 Zhijin white geese were measured, and 15 pairs of microsatellite primers with good polymorphism were selected from 52 pairs of microsatellite primers. There were 63 alleles detected; The average number of valid alleles was 2.066 5; The CKW21 locus contained the most valid alleles. The observed heterozygosity of each locus ranged from 0 to 0.969 7 and the expected heterozygosity ranged from 0.265 7 to 0.676 2, with the lowest expected heterozygosity value for locus Ans17 and the highest expected heterozygosity value for locus CKW21. The Shannon index ranged from 0.539 2 to 1.307 4, and the CKW21 locus had the highest Shannon's information index. The polymorphism information content of each locus ranged from 0.248 9 to 0.620 2, with the Ans17 locus having the lowest polymorphism information content and the CKW21 locus having the highest polymorphism information content. The mean value of the inbreeding coefficient was 0.197 2. The loci 5A5397, 5A265164, Ans21, Ans25, CKW10, CKW48, and TTUCG2 were found to deviate from Hardy-Weinberg equilibrium significantly by chi-square test. The correlation analysis revealed that in male geese, 5 loci were correlated with body weight, body oblique length, chest width, sternum length, pelvic width and shank length, respectively; In female geese, 10 loci were correlated with different body size indexes except body oblique length. The results of the study can provide some reference for the molecular marker-assisted breeding of Zhijin white goose.
GUO Zhi-Li,ZHAO Zhong-Long,YANG Hong, et al. Genetic Diversity of Microsatellite Markers in Zhijin White Geese (Anser cygnoides orientalis) and Its Association Analysis with Body Size Indexes[J]. 农业生物技术学报, 2023, 31(11): 2341-2357.
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