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农业生物技术学报  2023, Vol. 31 Issue (11): 2330-2340    DOI: 10.3969/j.issn.1674-7968.2023.11.011
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Effect of Chinese Herbal Compound Extract on Inflammation of Intestinal Epithelial Cells and Bacterial Diarrhea in Dogs (Canis lupus familiaris)
LIU Hai-Xia1, LIU Gen-Xin1,*, TIAN Qi-Hui1, ZHAO Tian2, ZHANG Jie-Hui1, ZHU Yan-Hua1, LIU Di-Qin1, GUO Zhi-Ming1
1 Gansu Polytechnic College of Animal Husbandry & Engineering, Wuwei 733006, China;
2 College of Veterinary Medicine, Gansu Agricultural University
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Abstract  Canine bacterial diarrhea is a common diarrhea disease caused by pathogenic bacteria in dogs (Canis lupus familiaris). Antibiotics are often used in clinical treatment, which also increases the incidence of drug-resistant bacteria. Qinlian Qinpi Decoction has good effect in the treatment of bacterial diarrhea in young children. In order to explore the therapeutic mechanism of Qinlian Qinpi Decoction in canine bacterial diarrhea, this study used semi-bionic enzyme extraction method to extract the effective components of Qinlian Qinpi Decoction, and the antibacterial effect of the extract was detected by punching method, the cytotoxicity was detected by Cell Counting Kit-8 (CCK-8), and dog intestinal epithelial cells were infected with Escherichia coli. The levels of Toll-like receptor 4 (TLR4), nuclear factor κB (NF-κB), interleukin 1β (IL1β), IL6, proliferating cell nuclear antigen (PCNA), B cell lymphoma-2 (Bcl2) and Bcl2-associated X (Bax) gene and protein were detected by qRT-PCR, Western blot and ELISA, and the cell apoptosis was detected by TUNEL method. Finally, the clinical therapeutic effect of the extract on canine bacterial diarrhea was determined by observation method. The results showed that the extract effectively inhibited the growth of E. coli in a certain concentration range and time without cytotoxicity. In canine intestinal epithelial cells infected with large intestine, the extract effectively inhibited the transcription levels and protein expression of TLR4 and NF-κB, and inhibited the transcription levels and cell secretion levels of IL-1β and IL-6. At the same time, the extract had obvious effects in anti-apoptosis and promoting cell proliferation. The results of in vivo experiments showed that the extract was effective for canine bacterial diarrhea, and when combined with ceftriaxone sodium, the treatment time could be shortened on the basis of effective treatment. The above results showed that the extract of Qinlian Qinpi Decoction could be applied to the treatment of bacterial diarrhea in dogs and helped to reduce the use of antibiotics in clinical treatment. This study provides basic data for the application of Qinlian Qinpi Decoction in the clinical treatment of bacterial diarrhea in dogs.
Key wordsQinlian Qinpi Decoction      Bacterial diarrhea in dogs      Toll-like receptor 4 (TLR4)      Nuclear factor κB (NF-κB)     
Received: 16 December 2022     
ZTFLH:  S859.3  
Corresponding Authors: *gsxmxgclgx@163.com   
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Articles by authors
LIU Hai-Xia
LIU Gen-Xin
TIAN Qi-Hui
ZHAO Tian
ZHANG Jie-Hui
ZHU Yan-Hua
LIU Di-Qin
GUO Zhi-Ming
Cite this article:   
LIU Hai-Xia,LIU Gen-Xin,TIAN Qi-Hui, et al. Effect of Chinese Herbal Compound Extract on Inflammation of Intestinal Epithelial Cells and Bacterial Diarrhea in Dogs (Canis lupus familiaris)[J]. 农业生物技术学报, 2023, 31(11): 2330-2340.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.11.011     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I11/2330
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