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| Pathogenicity Analysis of Papaya leaf curl Guangdong virus and Euphorbia leaf curl virus |
| LI Jing-Yuan1, LIN Wen-Zhong1, LI Ding-Shan1, MA Chong-Huan1, WU Zu-Jian1, DU Zhen-Guo1, ZHANG Jie1,2,* |
1 Institute of Plant Virology/State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China |
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Abstract In recent years, Papaya leaf curl Guangdong virus (PaLCuGDV) and Euphorbia leaf curl virus (EuLCV) were usually identified in Passiflora edulis. The full-length sequences of the 2 viruses, 2 732 and 2 745 bp respectively, were amplified from Passiflora edulis showing typical geminiviral damage symptoms in Fujian province. In order to determine the pathogenicity of PaLCuGDV and EuLCV, the infectious clones of 2 viruses were constructed by homologous recombination technology and Nicotiana benthamiana plants were inoculated with Agrobacterium-mediated method. The results showed that the symptoms of N. benthamiana inoculated with EuLCV were obviously dwarfed and the top leaves were deformed and curled, which was significantly heavier than that of PaLCuGDV, while the latter only showed slightly curly new leaves and yellowing leaves. Further more, the symptoms of mixed infection of the 2 viruses was much heavier than any of the symptoms induced by PaLCuGDV or EuLCV alone. Southern blot results showed that N. benthamiana inoculated with PaLCuGDV and EuLCV alone or in combination had high virus accumulation. In this study, the infectious clone of PaLCuGDV was constructed for the first time, and it was found that the mixed infection of EuLCV and PaLCuGDV could aggravate the induced symptoms. This study provides a reference for understanding the pathogenic mechanism of PaLCuGDV and EuLCV.
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Received: 07 October 2022
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Corresponding Authors:
* jiezhang3553@163.com
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