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农业生物技术学报  2023, Vol. 31 Issue (9): 1924-1934    DOI: 10.3969/j.issn.1674-7968.2023.09.013
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Effect of NbMVKa Interacting with AV2 Protein of Tomato leaf curl Hsinchu virus on Virus Pathogenicity
LI Ding-Shan1, HAN Xing1,2, LIN Wen-Zhong1, ZHA Qing-Chen1, ZHANG Wen-Wen1, DU Zhen-Guo1, ZHANG Jie1,*, WU Zu-Jian1
1 Institute of Plant Virology/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2 Jiangxi Agricultural Technology Promotion Center, Nanchang 330046, China
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Abstract  Monopartite geminiviral V2 protein plays an important role in the process of virus infection, while the research on the function of bipartite geminiviral AV2 protein is relatively few. To reveal the function of the AV2 protein of bipartite geminiviral Tomato leaf curl Hsinchu virus (ToLCHsV) by using the technique of proximity-dependent biotin identification (BioID)-mass spectrometry, several host proteins proximal to AV2 were preliminarily screened in Nicotiana benthamiana. The interaction between mevalonate kinase a (NbMVKa) and ToLCHsV-AV2 was verified by yeast two hybrid (YTH) and bimolecular fluorescence complementation (BiFC). Using the Tobacco rattle virus (TRV)-induced gene silencing technique, ToLCHsV infectious clone was inoculated in N. benthamiana plants. The results showed that 10 d post inoculation (dpi), NbMVKa silenced N. benthamiana plants showed no obvious symptoms, while the non-silenced plants showed leaf curl. Meanwhile, using the Southern blot analysis, the accumulation of viral DNA in silenced plants was relatively decreased. After 20 dpi, symptoms of NbMVKa silenced N. benthamiana plants were basically the same as those of the non-silenced plants. The results revealed that NbMVKa might play a positive role in regulating the pathogenesis of the virus by interacting with ToLCHsV-AV2. These results provide theoretical basis for further elucidating the function of bipartite geminiviral AV2 protein.
Key wordsTomato leaf curl Hsinchu virus      AV2 protein      Proximity-dependent biotin identification (BioID)      N. benthamiana mevalonate kinase a (NbMVKa)     
Received: 14 October 2022     
ZTFLH:  S432.1  
Corresponding Authors: * jiezhang3553@163.com   
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LI Ding-Shan
HAN Xing
LIN Wen-Zhong
ZHA Qing-Chen
ZHANG Wen-Wen
DU Zhen-Guo
ZHANG Jie
WU Zu-Jian
Cite this article:   
LI Ding-Shan,HAN Xing,LIN Wen-Zhong, et al. Effect of NbMVKa Interacting with AV2 Protein of Tomato leaf curl Hsinchu virus on Virus Pathogenicity[J]. 农业生物技术学报, 2023, 31(9): 1924-1934.
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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.09.013     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I9/1924
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