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| Isolation, Identification and Saline-alkaline Tolerance Mechanism Study of Arthrobacter rhombi Strain BFL-3 |
| LU Yu-Xin1,2, YAN Hong1,2, BAI Ya-Ni1,2*, LIANG Jian-Qiang1,2, FENG Zhi-Zhen1,2 |
1 Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China;
2 Enzyme Engineering Research Center of Shaanxi Province, Xi'an 710600, China |
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Abstract The resources of saline-alkaline tolerant microorganisms can promote soil nutrient circulation, enhance the water reservation and salinity tolerance ability of the plant, they have important application in remediation of saline-alkaline soil. Previously, a saline-alkaline resistant bacterial strain BFL-3 was isolated, the purpose of this study was to clarify the genetic evolution relationship, saline-alkaline tolerance and mechanism of the strain. The strain BFL-3 was identified by morphology and 16S rRNA sequence analysis. The growth characteristics of strain BFL-3 under different NaCl content and pH stresses were determined by optical density measurement. Besides, the whole genome of strain BFL-3 was sequenced, followed by gene function annotation and bioinformatics analysis. qPCR was applied to verify the expression of genes related to saline-alkaline tolerance of BFL-3 in medium containing 5% NaCl and pH 9. The results showed that the strain BFL-3 was identified as Arthrobacter rhombi, it could tolerant 0.5%~10% of NaCl, the tolerance range of pH value was 5~10. The strain contained 35 scaffolds, the total sequence length was 3 476 199 bp, G+C mol% was 69.15%, coding 3 170 genes, including 50 tRNA genes and 7 rRNA genes. Gene annotation showed that strain BFL-3 was enriched in a large number of genes in amino acid and carbohydrate metabolism pathways, and a certain number of genes in ion transporter related pathways, suggesting that the strain adopts 2 strategies of solute accumulation and ion balance in response to saline-alkaline stress. When the NaCl content was 5% and the pH value was 9, the expression level of trehalose synthase gene (treS), diaminobutyrate-2-oxoglutarate transaminase gene (ectB), glutamate dehydrogenase gene (gdhA), glycine betaine/L-proline ABC transporter permease gene (proW), Trk system potassium transporter gene (trkA) and choline ABC transporter permease gene (opuBD) were up-regulated, 11.0, 6.2, 6.0, 13.1, 7.4 and 6.3 times of the control group, respectively (P<0.01). These results indicated that strain BFL-3 could alleviate the saline-alkaline stress through the synthesis and accumulation of cytocompatible substances (such as trehalose, ectoine and glutamate) and regulating ion balance in the cell. This study clarified the saline-alkaline tolerance characteristics of BFL-3, explored its saline-alkaline tolerance mechanism, provided scientific basis for the further application of this strain in remediation of saline-alkaline soil.
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Received: 27 July 2022
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Corresponding Authors:
*baiyn@xab.ac.cn
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