用于灰茭鉴别的菰黑粉菌SNP位点挖掘及验证

doi:10.3969/j.issn.1674-7968.2023.02.016

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Abstract Jiaobai as an important aquatic vegetable in China, nearly 20% of the grey Jiaobai in the field has a serious impact on its yield. Previous research showed that the formation of 2 phenotypes of normal Jiaobai and grey Jiaobai in the field was closely related to the differentiation of MT and T type strains of Ustilago esculenta. Grey Jiaobai is caused by the infection of Zizania latifolia by T type strain of U. esculenta. Therefore, 129 494 SNP loci with difference between MT and T-type strains was obtained in this study, by resequencing, SNP calling and SNP filtration. After that, 647 specific SNP loci with specificity of T type of strain were screened by Hardy-Weinberg equilibrium test strategy and short fragment sieving. Finally, based on the 48 loci selected by randomization principle, 8 loci with high accuracy were screened by allele specific PCR (AS-PCR) with designed specific primers for field test. The results showed that the occurrence ratio of grey Jiaobai could be reduced 37.59% after eliminating the seedlings with positive identification results using the combination of the selected 8 specific loci to identify the T-type strain. This research provides technical support for further improving the purity of seedlings in improved seed breeding and subsequent seedling quality detection.

Key words： Jiaobai Ustilago esculenta SNP Allele specific PCR (AS-PCR) Grey Jioabai identification

Received: 16 January 2022

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Corresponding Authors: **zyfzjhzyh@163.com

About author:: * These authors contributed equally to this work

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	Articles by authors
	WU Jun-Cheng
	GE Xin-Tao
	YANG Meng-Fei
	YE Zi-Hong
	XIA Wen-Qiang
	TANG Jin-Tian
	YU Xiao-Ping
	ZHANG Shang-Fa
	ZHANG Ya-Fen

Cite this article:

WU Jun-Cheng,GE Xin-Tao,YANG Meng-Fei, et al. Mining and Verification of SNP Loci of Ustilago esculenta Used for Grey Jiaobai Identification[J]. 农业生物技术学报, 2023, 31(2): 383-392.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.02.016 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I2/383

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