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| Tissue Expression, Functional Prediction of CYP17A1 and CYP19A1 Genes in Qianbei Ma Goat (Capra hircus) and Its Association Analysis with Litter Size |
| ZHANG Yan1,2,3, CHEN Xiang1,2,3,*, ZHOU Zhi-Nan1,2,3, FU Kai-Bin1,2,3, WANG Zhong1,2,3, LI Shi-Jun1,2,3, YANG Pei-Fang1,2,3, HUI Mao-Mao1,2,3 |
1 Key Laboratory of Genetic Breeding and Breeding of Highland Mountain Animals, Ministry of Education, Guizhou University, Guiyang 550025, China;
2 Key Laboratory of Genetic Breeding and Breeding of Animals, Guizhou Province, Guiyang 550025, China;
3 School of Animal Science, Guizhou University, Guiyang 550025, China |
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Abstract CytochromeP450c17 (CYP17A1) and cytochromeP450 aromatase (CYP19A1) were important for follicle development, ovulation and the secretion and synthesis of major reproductive hormones, and affected the reproductive performance of animals. In this study, healthy Qianbei Ma goat (Capra hircus) were taken as the research object. The tissue expression of CYP17A1 and CYP19A1 genes was detected by qPCR, and the SNP mutation sites were screened by PCR direct sequencing. The effect of SNPs on the lambing number of different parity of Qianbei Ma goat was analyzed by establishing a general linear model. The expression of CYP17A1 and CYP19A1 genes in various tissues and the correlation between SNPs and litter size were analyzed. The results showed that the expression of CYP17A1 gene was the highest in the ovary of single and multiple lambs, and was extremely significantly higher than that of pituitary, hypothalamus, uterus and fallopian tube (P<0.01). CYP19A1 gene expression was higher in the multi-lamb ovary and uterus, and both were higher than the corresponding single-lamb tissue (P<0.01). A total of 7 SNPs were detected in the CYP17A1 gene coded region. g.59G>C, g.128G>A, g.129A>G and g.164T>C were all located in the first exon, g.2045G>A was in the second exon and g.4387G>A and g.4456C>T were in the sixth exon. Correlation analysis showed that the SNPs of CYP17A1 gene were not correlated with the litter size of Qianbei Ma goat. A mutation site g.48818C>A was detected in the 9th exon of the coding region of CYP19A1 gene and the number of lambs in the AA genotype was significantly higher than that of the CA genotype (P<0.05), but not significantly different from that of the CC genotype. The results showed that the expression of CYP17A1 and CYP19A1 genes in Qianbei Ma goat was the highest in the ovarian tissue of multiple lambs (P<0.01). There were only 4 goats in 150 with AA genotype g.48818C>A site, which was not representative and needs to be verified. This study provides basic data for further exploring the relationship between CYP17A1 and CYP19A1 gene variants and animal reproduction.
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Received: 23 August 2021
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Corresponding Authors:
* xchen2@gzu.edu.cn
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