|
|
|
| Identification of HSF and HSP Gene Family in Pomacea canaliculata and Their Expression Pattern Under Temperature Stress |
| CAO Meng-Yu, FAN Yue-Yuan, YIN Chuan-Lin*, YU Xiao-Ping* |
| College of Life Sciences/Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, China |
|
|
|
|
Abstract As a serious global invasive species with strong adaptability to the environment, the golden apple snail (Pomacea canalicata) had spread to 16 provinces cities and regions in the southern China. Heat shock proteins (HSPs) are an important type of molecular chaperone proteins produced by organisms in response to environmental stress and environmental factor stimulation can induce its expression to cope with adverse effects of organism; the heat shock factor (HSF) is a key factor of transcription regulating of heat shock protein genes. In order to explore the role of HSF and HSP genes in the regulation of temperature tolerance of snails, in this study, only 1 HSF1 gene were identified by bioinformatics methods from the P. canaliculata as well as other 3 apple snails (P. maculata, Lanistes nyassanus, Marisa cornuarietis) which were in the same genus or family, and also 63, 66, 67, 64 HSP genes were identified, respectively. The amino acid length and gene structure of HSF1 gene in 4 snails were similar and highly conservative, which showed low gene diversity during the evolution of species. The HSP genes in the 4 snails had little difference, but the subfamily composition of the gene family was quite different. The HSP90 gene was relatively conserved, while HSP20 gene changed greatly. The HSP40 gene had the most members in the gene family among the 4 apple snails. Based on the gene expression of RNA-seq data, the results showed that the expression of HSF1 gene from high to low were gill, foot muscle, liver and ovary, respectively. Under high temperature stress, the HSF1 gene expression in 4 tissues were highly expressed, and the expression pattern was consistent in foot muscle and liver tissue. According to the HSP gene expression heat map, 17 HSP genes were specifically highly expressed under high temperature stress, results of 12 genes were consistent with the qPCR. The present study provides a reference basis for exploring the mechanism of HSF and HSP gene family in temperature tolerance.
|
|
Received: 16 March 2022
|
|
|
|
Corresponding Authors:
*chuanlinyin@cjlu.edu.cn;yxp@cjlu.edu.cn
|
|
|
|
[1] 陈宝雄, 孙玉芳, 韩智华, 等. 2020. 我国外来入侵生物防控现状、问题和对策[J]. 生物安全学报, 29(03): 157-163.
(Chen B X, Sun Y F, Han Z H, et al.2020. Challenges in preventing and controlling invasive alien species in China[J]. Biosafety, 29(03): 157-163.)
[2] 董胜张, 白旭, 潘颖瑛, 等. 2010. 温度胁迫对我国不同地理种群福寿螺生长及存活的影响[J]. 湖北农业科学, 49(11): 2878-2882.
(Dong S Z, Bai X, Pan Y Y, et al.2010. Effects of temperature stress on development and survival rate of the apple snail, Pomacea canaliculata(Lamarck) from different geographical populations in China[J]. Hubei Agricultural Sciences, 49(11): 2878-2882.)
[3] 周宇, 袁雪颖, 杨子轩,等. 2018. 福寿螺入侵中国的扩散动态及潜在分布[J]. 湖泊科学, 30(05): 1379-1387.
(Zhou Y, Yuan X Y, Yang Z X, et al.2018. Dispersal dynamics and potential distribution of the invasion of Ampelopsis japonicus in China[J]. Lake Science, 30(05): 1379-1387.)
[4] 周卫川, 吴宇芬, 杨佳琪. 2003. 福寿螺在中国的适生性研究[J]. 福建农业学报, 18(01): 25-28.
(Zhou W C, Wu Y F, Yang J Q.2003. Viability of the ampullaria snail in China[J]. Fujian Journal of Agricultural Science, 28(01): 25-28)
[5] Abascal F, Zardoya R, Posada D.2005. ProtTest: Selection of best-fit models of protein evolution[J]. Bioinformatics, 21(9): 2104-2105.
[6] Bailey T L, Johnson J, Grant C E, et al.2015. The meme suite[J]. Nucleic Acids Research, 43(W1): W39-W49.
[7] Beckmann R P, Lovett M, Welch W J.1992. Examining the function and regulation of hsp 70 in cells subjected to metabolic stress[J]. Journal of Cell Biology, 117(6): 1137-1150.
[8] Carlsson N, Kestrup Å, Mårtensson M, et al.2004. Lethal and non‐lethal effects of multiple indigenous predators on the invasive golden apple snail (Pomacea canaliculata)[J]. Freshwater Biology, 49(10): 1269-1279.
[9] Chen S, Zhou Y, Chen Y, et al.2018. Fastp: An ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 34(17): i884-i890.
[10] Clos J, Westwood J T, Becker P B, et al.1990. Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation[J]. Cell, 63(5): 1085-1097.
[11] Fujimoto M, Hayashida N, Katoh T, et al.2010. A novel mouse HSF3 has the potential to activate nonclassical heat-shock genes during heat shock[J]. Molecular Biology of the Cell, 21(1): 106-116.
[12] Giraud-Billoud M, Vega IA, Tosi M E R, et al.2013. Antioxidant and molecular chaperone defences during estivation and arousal in the South American apple snail Pomacea canaliculate[J]. Journal of Experimental Biology, 216(Pt 4): 614-622.
[13] Hayes K A, Burks R L, Castro-Vazquez A, et al.2015. Insights from an integrated view of the biology of apple snails (Caenogastropoda: Ampullariidae)[J]. Malacologia, 58(1-2): 245-302.
[14] Hu B, Jin J P, Guo A Y, et al.2015. GSDS 2.0: An upgraded gene feature visualization server[J]. Bioinformatics, 31(8): 1296-1297.
[15] Jacob P, Hirt H, Bendahmane A.2017. The heat-shock protein/chaperone network and multiple stress resistance[J]. Plant Biotechnology Journal, 15(4): 405-414.
[16] Johnson L S, Eddy S R, Portugaly E.2010. Hidden Markov model speed heuristic and iterative HMM search procedure[J]. BMC Bioinformatics, 11: 431.
[17] Katoh K, Standley D M.2013. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability[J]. Molecular Biology and Evolution, 30(4): 772-780.
[18] Kim D, Paggi J M, Park C, et al.2019. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype[J]. Nature Biotechnology, 37(8): 907-915.
[19] Lee S B, Lee S M, Park Chung Bae, et al.2019. The environmental adaptability of Pomacea canaliculata used for weed control in wet rice paddies and crop damage caused by overwintered golden apple snails[J]. Korean Journal of Environmental Agriculture, 38(1): 23-33.
[20] Lin Y X, Jiang H Y, Chu Z X, et al.2011. Genome-wide identification, classification and analysis of heat shock transcription factor family in maize[J]. BMC Genomics, 12:76.
[21] Liu G F, Yang Q Q, Lin H F, et al.2018a. Differential gene expression in Pomacea canaliculata (Mollusca: Gastropoda) under low temperature condition[J]. Journal of Molluscan Studies, 397-403.
[22] Liu C, Zhang Y, Ren Y, et al.2018b. The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation[J]. GigaScience, 7(9): giy101.
[23] Liu J, Sun Z, Wang Z, et al.2020a. A comparative transcriptomics approach to analyzing the differences in cold resistance in Pomacea canaliculata between Guangdong and Hunan[J]. Journal of Immunology Research, 2020(1): 1-9.
[24] Liu Y, Li L, Qi H, et al.2020b. Regulation between HSF1 isoforms and HSPs contributes to the variation in thermal tolerance between two Oyster congeners[J]. Frontiers in Genetics, 11: 581725.
[25] Lowe S, Browne M, Boudjelas S, et al.2000. 100 of the world's worst invasive alien species: A selection from the global invasive species database[M]. Auckland: Invasive Species Specialist Group, New Zealand.
[26] Meher P K, Sahu T K, Gahoi S, et al.2018. ir-HSP: Improved recognition of heat shock proteins, their families and sub-types based on g-spaced di-peptide features and support vector machine[J]. Frontiers in Genetics, 8: 235.
[27] Nover L, Bharti K, Döring P, et al.2001. Arabidopsis and the heat stress transcription factor world: How many heat stress transcription factors do we need[J]? Cell Stress & Chaperones, 6(3): 177-189.
[28] Pertea M, Pertea G M, Antonescu C M, et al.2015. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads[J]. Nature Biotechnology, 33(3): 290-295.
[29] Song H M, Mu X D, Gu D E, et al.2014. Molecular characteristics of the HSP70 gene and its differential expression in female and male golden apple snails (Pomacea canaliculata) under temperature stimulation[J]. Cell Stress & Chaperones, 19(4): 579-589.
[30] Sun J, Mu H, Ip J, et al.2019. Signatures of divergence, invasiveness, and terrestrialization revealed by four apple snail genomes[J]. Molecular Biology and Evolution, 36(7): 1507-1520.
[31] Voorrips R.2002. MapChart: Software for the graphical presentation of linkage maps and QTLs[J]. The Journal of Heredity, 93(1): 77-78.
[32] Vos MJ, Hageman J, Carra S, et al.2008. Structural and functional diversities between members of the human HSPB, HSPH, HSPA, and DNAJ chaperone families[J]. Biochemistry, 47(27): 7001-7011.
[33] Wiederrecht G, Seto D, Parker C S.1988. Isolation of the gene encoding the S. cerevisiae heat shock transcription factor[J]. Cell, 54(6): 841-853.
[34] Xu Y P, Zheng G, Dong S, et al.2014. Molecular cloning, characterization and expression analysis of HSP60, HSP70 and HSP90 in the golden apple snail, Pomacea canaliculate[J]. Fish & Shellfish Immunology, 41(2): 643-653.
[35] Xu Y P, Zheng G, Liu G F, et al.2019. Molecular cloning, characterization of Pomacea canaliculata HSP40 and its expression analysis under temperature change[J]. Journal of Thermal Biology, 81: 59-65.
[36] Yang Q Q, Liu S W, He C, et al.2018. Distribution and the origin of invasive apple snails, Pomacea canaliculata and P. maculata (Gastropoda: Ampullariidae) in China[J]. Scientific Reports, 8(1), 1185.
[37] Yang T B, Wu Z D, Lun Z R.2013. The apple snail Pomacea canaliculata, a novel vector of the rat lungworm, Angiostrongylus cantonensis: Its introduction, spread, and control in China[J]. Hawai'i Journal of Medicine & Public Health, 72(6 Suppl 2): 23-25. |
|
|
|
