Response of hsp90b1 and hspb1 to Temperature Stress in the Liver of Larimichthys polyactis
CHU Tian-Qi1,2, LIU Feng1,*, CHEN Hong-Lin1, ZHAN Wei1, WANG Meng-Jie1,2, QIN Gao-Chan1,2, LOU Bao1,*, XU Wan-Tu3
1 Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 China; 2 School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China; 3 Xiangshan Harbor Aquatic Seed Co., Ltd., Ningbo 315700, China
Abstract Heat shock proteins (HSPs) are closely related to fish response to temperature stress. To investigate the response characteristics of the heat shock proteins family genes, hsp90b1 and hspb1, in Larimichthys polyactis subjected to temperature stress, In this study, full-length CDS of hsp90b1 and hspb1 in L. polyactis was cloned, and detected their spatiotemporal expression characteristics and expression patterns after acute and long-term temperature stress. The full-length CDS sequence of hsp90b1 (GenBank No. OK340652) was 2 406 bp, encoding a predicted 801 amino acid. While the full-length CDS sequence of hspb1 (GenBank No. OK340653) was 609 bp encoding 202 amino acid. Tissue expression analysis results showed that the hsp90b1 gene expression is the highest in the skin, while hspb1 gene expression was the highest in the muscle. As the natural water temperature was 20 ℃ (control group), L. polyactis was treated with acute high (32 ℃) and low (6 ℃) temperature stress. The results showed that the acute temperature stress could induce significant high expression of hsp90b1 in the liver. Differently, hspb1 expressed higher with high temperature and expressed lower with low temperature (P<0.05). During long-term temperature treatments (with 13 ℃ as the control, fish was treated at 6, 8, 16 and 20 ℃, respectively and maintained for 7 d). It was found that the expression of hsp90b1 in the liver of the 4 treatment groups was significantly higher than that in the control group, which temperature was 13 ℃ (P<0.05), while the expression level of the high-temperature group (16 ℃ and 20 ℃) was higher than that in the low-temperature group (6 ℃ and 8 ℃); Unlike hsp90b1, hspb1 still showed the characteristics of high expression at high temperature and low expression at low temperature (P<0.05). The present study indicated that hsp90b1 and hspb1 were significantly differentially expressed in L. polyactis during temperature stress, suggested that hsp90b1 and hspb1 played an important role in the response to temperature stress. The results of this study provide an important foundation for exploring the molecular mechanism of the L. polyactis in response to temperature stress.
CHU Tian-Qi,LIU Feng,CHEN Hong-Lin, et al. Response of hsp90b1 and hspb1 to Temperature Stress in the Liver of Larimichthys polyactis[J]. 农业生物技术学报, 2022, 30(3): 528-538.
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