Screening and Functional Prediction of Candidate Target Genes Regulated by Transcription Factor StMR1 of Setosphaeria turcica
YIN Yu-Juan1,*, LI Hai-Xiao1,2,*, WANG Qiu-Yue1,2, JIA Hui2, LIU Ning1,2, DONG Jin-Gao1,2,**, CAO Zhi-Yan1,2,**
1 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China; 2 State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, China
AbstractSetosphaeria turcica melanin regulation factor 1 (StMR1) is a melanin transcriptional regulatory factor with a double zinc finger domain in Setosphaeria turcica, which affects the growth, metabolism and pathogenicity by regulating gene expression. Therefore, it is very important to elucidate the regulation mode of this transcription factor for analyzing the pathogenic mechanism of S. turcica. The previous study of our group found that after the StMR1 gene was knocked out, the synthesis of melanin in the mutant was blocked, the colony changed from black to gray-white, and the pathogenicity was significantly reduced. In this study, the RNA sequence (RNA-seq) data of wild-type strains of S. turcica and StMR1 gene deletion mutants were used to screen differentially expressed genes (GEGs). Gene Ontology (GO) and KEGG function annotation analysis were conducted for DEGs. The screening obtained 1 383 up-regulated genes and 1 710 down-regulated genes. The down-regulated genes were related to molecular functions, cellular components and biological processes, involving 25 metabolic pathways in 6 categories. Six down-regulated genes, SETTUDRAFT_172122, SETTUDRAFT_168776, SETTUDRAFT_166944, SETTUDRAFT_162601, SETTUDRAFT_131269 and SETTUDRAFT_163249, were analyzed of the expression level by qRT-PCR. The qRT-PCR results showed that the expression levels of the 6 genes were down-regulated in the mutant, suggested that StMR1 might have a positive regulation effect on the genes. Bioinformatics analysis showed that gene SETTUDRAFT_162601 had a typical Catalase domain, and the expression level of SETTUDRAFT_162601 was significantly increased under H2O2 by qRT-PCR (P<0.05), suggested that this gene was related to antioxidant stress. These results provide reference for further study on the mechanism of transcription factor StMR1 regulating pathogenicity, and also provide a reference for analyzing the function of zinc finger transcription factors.
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