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| Metabolome Analysis of Effect of Light Intensity Stress on Metabolism of Golden Pompano (Trachinotus ovatus) |
| ZHONG Zhi-Ming1,2,*, CHEN Jia-Yu1,2,*, ZHANG Jing1,2, TANG Bao-Gui1,2, YU Fei-Fei1,2,**, LAI Wen-Qi2, ZHU Jie-Xiong2 |
1 South Marine Science and Engineering Guangdong Provincial Laboratory (Zhanjiang), Zhanjiang 524006, China;
2 Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China |
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Abstract Light intensity is an important environmental factor that can affect the physiological behavior and metabolism of cultured varieties in aquaculture. In this study, 3 light intensities were used to stress the golden pompano (Trachinotus ovatus), including low light intensity (10 lx), medium light intensity (about 250 lx), and high light intensity (1250 lx). The metabolome analysis was performed to illustrate the effect of extreme lights on metabolism of T. ovatus. The liquid chromatography-mass spectrometry (LC/MS) was employed to detect the metabolites. The principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) were used to screen differential metabolites in the liver, and the MetaboAnalyst database was used to analyze metabolism-related pathways. The results showed that compared with the control group, 102 differential metabolites in 10 lx group were found and enriched in 39 metabolic pathways, including β-alanine metabolism, tyrosine metabolism, L-glutamate metabolism and biosynthesis of unsaturated fatty acids. 55 differential metabolites in 1 250 lx group were found and enriched in 27 metabolic pathways, including glucose metabolism, biotin metabolism, thiamine metabolism, pyruvate metabolism and taurine and hypotaurine metabolism. Pathway analysis showed that low light stress inhibited amino acid metabolism and fatty acid synthesis in T. ovatus, and the fish might try to adapt low light environment by reducing movement, enhancing immune function and antioxidant capacity. The high light stress promoted glucose production, inhibited pyruvate metabolism and taurine synthesis, and might affect metabolism and immune function of T. ovatus. This study provides a valuable reference for setting culture parameters in T. ovatus aquaculture.
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Received: 29 June 2021
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Corresponding Authors:
**yufeifei2000@163.com
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| About author:: * These authors who contributed equally to this work |
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