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| Baicalin Regulates Heat Stress-induced Uterine Oxidative Damage in Mice (Mus musculus) via Nrf2/Keap1 Signaling Pathway |
| GAO Shan-Song, WANG Lei, LI Hua-Tao, CAO Rong-Feng, CONG Xia, TIAN Wen-Ru, FENG Yan-Ni*, JIANG Zhong-Ling* |
| College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China |
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Abstract Mammal's reproductive function is the most vulnerable to heat stress in all physiological functions. The main objectives of this research were to explore the influence of baicalin on oxidative damage of uterus tissue in mice (Mus musculus), and understand the anti-oxidation principle of baicalin. Female mice were divided into control (C) group, baicalin (Bai) group, heat stress (H) group and baicalin plus heat stress (H+Bai) group. TUNEL, spectrophotometry, Western blot and immunohistochemistry were used to detect uterine tissue structure, apoptosis and signal transduction. The results showed that, compared with the H group, the uterine tissue injury and apoptosis in mice treated with heat stress were reduced by baicalin, the content of MDA (malondialdehyde) was extremely significantly decreased (P<0.01), the activity of T-SOD (total superoxide dismutase dismutase) was extremely significantly increased (P<0.01), the activity of CAT(catalase) and GSH-Px (glutathione peroxidase) was significantly increased (P<0.05) in group H+Bai. The expression levels of Keap1 (kelch-like ECH-associated protein-1) and Nrf2 (NF-E2-related factor 2) proteins were remarkably decreased by baicalin (P<0.05), and the expressions of Bcl-2 (B-cell lymphoma-2), Bax (Bcl-2 associated X protein), Apaf-1 (apoptotic protease activating factor-1), Caspase-9 (cysteinyl aspartate specific proteinase-9) and Caspase-3 proteins in endometrial epithelial cells and adenocarcinoma epithelial cells were decreased in group H+Bai. Overall, baicalin might attenuate oxidative damage and apoptosis in endometrial epithelial cells of heat-stressed mice by activating Keap1/Nrf2 signaling pathway and then regulating the activity of antioxidant enzymes. The results of this research provide a theoretical approach to addressing animal heat stress, and provide basic information for screening anti-heat stress drugs and solving the decline of fecundity in summer dairy cattle (Bos taurus).
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Received: 24 April 2019
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Corresponding Authors:
yannifeng1979@hotmail.com; jzhl200666@126.com
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