|
|
|
| Effect of Transport Stress on Pathological Injury and Heat Shock Protein Expression of Liver and Kidney in Goats (Capra hircus) |
| ZHENG Wen-Ya1,2, ZHANG Zhi-Tao2, MEI Ting2, LIU Ben2, HU Wei2, CUI Yan1,* |
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2 College of Life Science and Resources and Environment/Laboratory for Disease Control of Beef Cattle and Sheep, Yichun University, Yichun 336000, China |
|
|
|
|
Abstract As the demand for goat (Capra hircus) products increased, the long-distance transportation of goats increased, and the transport stress would cause weight and meat quality decline, immunity decline, disease and even death of goats, and the management of goats during the transport would affect the profitability and animal welfare. The effects of transport stress on the microstructure and ultrastructure as well as the expression of heat shock proteins (HSP) of liver and kidney of goats were investigated. 12 healthy male goats from West Jiangxi were randomly divided into control group, 2 h transport stress group and 6 h transport stress group. HE staining, transmission electron microscopy, immunohistochemistry and Western blot were used to analyze the degree of injury and the distribution and expression of HSP27, HSP70 and HSP90 of liver and kidney in goats at different transport time. The results of microscopic and ultramicroscopic observation showed that different degrees of injury of the liver and kidney in the transportation stress group occurred. In the liver, hepatocytes showed obvious granule and vesicular degeneration, and part of chromatin aggregated in cell nuclei. In the kidney, the structure of renal tubules and collecting tubules were collapsed, the glomeruli were hyperemic and swollen, and the renal capsule lumen was narrowed. Moreover, the mitochondria in the cells of liver and kidney were both swollen and deformed, and the cristae was fractured and disappeared. The number of mitochondria was reduced, and some mitochondria even underwent membrane structure rupture due to swelling and vacuolation. The lesions of 6 h transport stress group were more serious, some liver cells showed nuclear lysis, and the damage degree of lumen and glomerular cells was also more serious. Immunohistochemical results showed that 3 HSP were mainly expressed in the cytoplasm of hepatocytes near the central vein and portal area, while in the kidney, the expressions were mainly in the renal tubules and collecting tubules, and the expressions were stronger in the transport group. In addition, HSP27 also showed strong positive results in vascular endothelium and bile duct cells in the liver and renal corpuscles in the transport group. Western blot results showed that there were no significant differences in the expression of these 3 HSPs in the liver and kidney between 2 and 6 h transport stress group (P>0.05), and the expression of HSP90 in liver between transport group and control group was also no significant differences (P>0.05). The expressions of HSP27 and HSP70 in liver in 2 and 6 h transport stress groups were extremely significantly (P<0.01) and significantly (P<0.05) higher than the control group, respectively. In the kidney, the expressions of HSP27 and HSP90 in 2 and 6 h transportation stress groups were both extremely significantly (P<0.01) higher than the control group, and the expressions of HSP70 in 2 and 6 h transport stress groups were significantly (P<0.05) and extremely significantly (P<0.01) higher than the control group, respectively. In conclusion, transport stress caused severe pathological damages to the liver and kidney of goats, and the damages worsened with the extension of transport time. These 3 HSP were distributed differently in liver and kidney. The expression levels of HSP27 and HSP70 in goat liver and kidney were both significantly increased after transport stress, which improved the stress resistance of hepatorenal cells. The results provide theoretical reference for the further study of solutions of goat transport stress.
|
|
Received: 22 March 2019
|
|
|
|
Corresponding Authors:
cuiyan369@sina.com
|
|
|
|
1 鲍恩东, Sultan K R, Nowak B, 等. 2002. 运输应激猪肝脏中热应激蛋白的定位与表达[J]. 中国农业科学, 35(9): 1130-1133.
(Bao E D, Sultan K R, Nowak B, et al.2002. Localization and expression of heat shock proteins in liver of transport stressed pigs[J]. Scientia Agricultura Sinica, 35(9): 1130-1133.)
2 Alcalde M J, Suárez M D, Rodero E, et al.2017. Effects of farm management practices and transport duration on stress response and meat quality traits of suckling goat kids[J]. Animal, 11(9): 1626-1635.
3 Bao E, Sultan K R, Nowak B, et al.2008. Expression and distribution of heat shock proteins in the heart of transported pigs[J]. Cell Stress and Chaperones, 13(4): 459-466.
4 Bing C, Feder M E, Le K.2018. Evolution of heat-shock protein expression underlying adaptive responses to environmental stress[J]. Molecular Ecology, 27(15): 3040-3054.
5 Biobaku K T, Omobowale T O, Akeem A O, et al.2018. Use of goat interleukin-6, cortisol, and some biomarkers to evaluate clinical suitability of two routes of ascorbic acid administration in transportation stress[J]. Veterinary World, 11(5): 674-680.
6 Cedraz H, Gracielle J, Gromboni G, et al.2017. Heat stress induces expression of HSP genes in genetically divergent chickens[J]. PLOS ONE, 12(10): e0186083.
7 Chen S W C, Park S W, Kim M, et al.2009. Human heat shock protein 27 overexpressing mice are protected against hepatic ischemia and reperfusion injury[J]. Transplantation, 87(10): 1478-1487.
8 De la Fuente J, Sánchez M, Pérez C, et al.2010. Physiological response and carcass and meat quality of suckling lambs in relation to transport time and stocking density during transport by road[J]. Animal, 4(02): 250-258.
9 Efthymiou C A, Mocanu M M, De Belleroche J, et al.2004. Heat shock protein 27 protects the heart against myocardial infarction[J]. Basic Research in Cardiology, 106(3): 392-394.
10 Fisher A D, Niemeyer D O, Lea J M, et al.2010. The effects of 12, 30, or 48 hours of road transport on the physiological and behavioral responses of sheep[J]. Journal of Animal Science, 88(6): 2144-2152.
11 Fujigaki Y, Sun Y, Fujikura T, et al.2010. Immunohistochemical study of heat shock protein 27 with respect to survival and regeneration of proximal tubular cells after uranyl acetate-induced acute tubular injury in rats[J]. Renal Failure, 32(1): 119-125.
12 Gaestel M, Gross B, Benndorf R, et al.2010. Molecular cloning, sequencing and expression in Escherichia coli of the 25-kD growth-related protein of ehrlich ascites tumor and its homology to mammalian stress proteins[J]. European Journal of Biochemistry, 179(1): 209-213.
13 Giridharan V V, Thandavarayan R A, Arumugam S, et al.2015. SchisandrinB ameliorates icv-infused amyloid β induced oxidative stress and neuronal dysfunction through inhibiting rage/nf-κb/mapk and up-regulating hsp/beclin expression[J]. PLOS ONE, 10(11): e0142483.
14 Guo Q, Du X, Zhao Y, et al.2014. Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats[J]. Molecular Medicine Reports, 10(6): 2875-2881.
15 Gupta S, Earley B, Crowe M A.2007. Effect of 12-hour road transportation on physiological, immunological and haematological parameters in bulls housed at different space allowances[J]. Veterinary Journal, 173(3): 605-616.
16 Ha K, Fiskus W, Rao R, et al.2011. Hsp90 inhibitor-mediated disruption of chaperone association of ATR with Hsp90 sensitizes cancer cells to DNA damage[J]. Molecular Cancer Therapeutics, 10(7): 1194-1206.
17 Haslbeck M.2002. sHsps and their role in the chaperone network[J]. Cellular & Molecular Life Sciences, 59(10):1649-1657.
18 Kadim I T, Mahgoub O, Al-Kindi A, et al.2006. Effects of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics of three breeds of Omani goats[J]. Meat Science, 73(4): 626-634.
19 Kozlov AV, Duvigneau JC, Hyatt TC, et al.2010. Effect of estrogen on mitochondrial function and intracellular stress markers in rat liver and kidney following trauma-hemorrhagic shock and prolonged hypotension[J]. Molecular Medicine (Cambridge, Mass.), 16(7-8): 254-261.
20 Lanneau D, Thonel A D, Maurel S, et al.2007. Apoptosis versus cell differentiation: Role of heat shock proteins HSP90, HSP70 and HSP27[J]. Prion, 1(1): 53.
21 Li F.2002. Heat stress prevents mitochondrial injury in ATP-depleted renal epithelial cells[J]. American Journal of Physiology-cell Physiology, 283(3): C917.
22 Li L, Zhang T, Zhou L, et al.2013. Schisandrin B attenuates acetaminophen-induced hepatic injury through heat-shock protein 27 and 70 in mice[J]. Journal of Gastroenterology and Hepatology, 29(3): 640-647.
23 Madrigalmatute J, Oscar L, Blancocolio L M, et al.2010. Heat shock protein 90 inhibitors attenuate inflammatory responses in atherosclerosis[J]. Atherosclerosis Supplements, 10(2): 330.
24 Marques A T, Lecchi C, Grilli G, et al.2016. The effect of transport stress on turkey (Meleagris gallopavo) liver acute phase proteins gene expression[J]. Research in Veterinary Science, 104: 92-95.
25 Masmeijer C, Devriendt B, Rogge T, et al.2019. Randomized field trial on the effects of body weight and short transport on stress and immunevariables in 2- to 4-week-old dairy calves[J]. Journal of Veterinary Internal Medicine, 33(3): 1514-1529.
26 Merchant S, Korbelik M.2011. Heat shock protein 70 is acute phase reactant: Response elicited by tumor treatment with photodynamic therapy[J]. Cell Stress & Chaperones, 16(2): 153-162.
27 Multhoff G, Schmid T E.2012. Radiation-induced stress proteins - the role of heat shock proteins (HSP) in anti-tumor responses[J]. Current Medicinal Chemistry, 19(12):1765-1770.
28 Myung S J, Yoon J H, Kim B H, et al.2009. Heat shock protein 90 inhibitor induces apoptosis and attenuates activation of hepatic stellate cells[J]. Journal of Pharmacology and Experimental Therapeutics, 330(1): 276-282.
29 Parcellier A, Gurbuxani S, Schmitt E, et al.2003. Heat shock proteins, cellular chaperones that modulate mitochondrial cell death pathways[J]. Biochemical and Biophysical Research Communications, 304(3): 505-512.
30 Pieper M, Rupprecht H D, Bruch K M, et al.2001. Requirement of heat shock protein 90 in mesangial cell mitogenesis[J]. Kidney International, 58(6): 2377-2389.
31 Ramírez V, Mejía-Vilet J M, Hernández D, et al.2008. Radicicol, a heat shock protein 90 inhibitor, reduces glomerular filtration rate[J]. American Journal of Physiology. Renal Physiology, 295(4): 1044-1051.
32 Rice J W, Veal J M, Fadden R P, et al.2014. Small molecule inhibitors of Hsp90 potently affect inflammatory disease pathways and exhibit activity in models of rheumatoid arthritis[J]. Arthritis & Rheumatology, 58(12): 3765-3775.
33 Rokutan K.2000. Role of heat shock proteins in gastric mucosal protection[J]. Journal of Gastroenterology and Hepatology, 15Suppl: D12-19.
34 Ruan G P, Xu F, Li Z A, et al.2013. Induced autologous stem cell transplantation for treatment of rabbit renal interstitial fibrosis[J]. PLOS ONE, 8(12): e83507.
35 Sanchez-Nino M D, Sanz A B, Sanchez-Lopez E, et al.2012. HSP27/HSPB1 as an adaptive podocyte antiapoptotic protein activated by high glucose and angiotensin Ⅱ[J]. Laboratory Investigation, 92(1): 32-45.
36 Shen B, Yuan Y, Cheng J, et al.2016. Activation of chronic toxoplasmosis by transportation stress in a mouse model[J]. Oncotarget,7(52): 87351.
37 Simova V, Voslarova E, Vecerek V, et al.2017. Effects of travel distance and season of the year on transport-related mortality in cattle[J]. Animal Science Journal, 88(3):526-532.
38 Sun F, Zuo Y Z, Ge J, et al.2018. Transport stress induces heart damage in newly hatched chicks via blocking the cytoprotective heat shock response and augmenting nitric oxide production[J]. Poultry Science, 97(8):2638-2646.
39 Tatsuki M, Madoka U, Haruna I, et al.2015. Small heat shock protein beta-1 (HSPB1) is upregulated and regulates autophagy and apoptosis of renal tubular cells in acute kidney injury[J]. PLOS ONE, 10(5): e0126229.
40 Teke B, Akdag F, Ekiz B, et al.2014. Effects of different lairage times after long distance transportation on carcass and meat quality characteristics of Hungarian Simmental bulls[J]. Meat Science, 96(1): 224-229.
41 Trepel J, Mollapour M, Giaccone G, et al.2010. Targeting the dynamic HSP90 complex in cancer[J]. Nature Reviews Cancer, 10(8): 537-549.
42 Tsai Y C, Lee Y M, Lam K K, et al.2013. The role of heat shock protein 70 in the protective effect of YC-1 on β-amyloid-induced toxicity in differentiated PC12 cells[J]. PLOS ONE, 8(7): e69320.
43 Van E W, Ruurd V D Z, Prakken B.2005. Heat-shock proteins induce T-cell regulation of chronic inflammation[J]. Nature Reviews Immunology, 5(4): 318-330.
44 Vidyasagar A, Wilson N A, Djamali A.2012. Heat shock protein 27 (HSP27): Biomarker of disease and therapeutic target[J]. Fibrogenesis& Tissue Repair, 5(1): 7.
45 Wang X, Chen M, Zhou J, et al.2014. HSP27, 70 and 90, anti-apoptotic proteins, in clinical cancer therapy (Review)[J]. International Journal of Oncology, 45(1): 18-30.
46 Wang Z, Gall J M, Bonegio R G B, et al.2011. Induction of heat shock protein 70 inhibits ischemic renal injury[J]. Kidney International, 79(8): 861-870.
47 Wolf J H, Bhatti T R, Fouraschen S, et al.2014. Heat shock protein 70 is required for optimal liver regeneration after partial hepatectomy in mice[J]. Liver Transplantation, 20(3): 376-385.
48 Xing T, Xu X L, Zhou G H, et al.2015.The effect of transportation of broilers during summer on the expression of heat shock protein 70, postmortem metabolism and meat quality[J]. Journal of Animal Science, 93(1): 62-70.
49 Xin T, Lei Z, Xianjing S, et al.2016. HSP27 inhibits homocysteine-induced endothelial apoptosis by modulation of ROS production and mitochondrial caspase-dependent apoptotic pathway[J]. BioMed Research International, 2016: 4847874.
50 Ye S Y, Wu J, Zhang J, et al.2010. Locally synthesized HSP27 in hepatocytes: Is it possibly a novel strategy against human liver ischemia/reperfusion injury?[J]. Medical Hypotheses, 76(2): 296-298.
51 Yilmaz A, Gedikbasi A, Yuruk Y Z, et al.2016. Higher urine heat shock protein 70/creatinine ratio in type 1 diabetes mellitus[J]. Renal Failure, 38(3): 404-410.
52 Yong Y H, Wang P, Jia R M, et al.2018. SOCS3 control the activity of NF-κB induced by HSP70 via degradation of MyD88-adapter-like protein (Mal) in IPEC-J2 cells[J]. International Journal of Hyperthermia, 36(1): 1-9.
53 Yu H, Bao E D, Zhao R Q, et al.2007. Effect of transportation stress on heat shock protein 70 concentration and mRNA expression in heart and kidney tissues and serum enzyme activities and hormone concentrations of pigs[J]. American Journal of Veterinary Research, 68(11): 1145-1150.
54 Zhong R Z, Liu H W, Zhou D W, et al.2011. The effects of road transportation on physiological responses and meat quality in sheep differing in age[J]. Journal of Animal Science, 89(11): 3742-3751. |
|
|
|
