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| Association Between Polymorphism of MHC-DQB2 Gene Exon 2 and Susceptibility to Coccidia in Shanbei White Cashmere Goats (Capra hircus) |
| SONG Xiao-Yue, SHI Lei, LIU Jin-Wang, ZHU Hai-Jing, LI Long-Ping, QU Lei |
1 Shaanxi Province Engineering & Technology Research Center of Shanbei Cashmere Goats/Yulin University, Yulin 719000, China;
2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
3 Shaanxi Province Jia County Tong Town Animal Husbandry & Veterinary Station, Yulin 719200, China; |
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Abstract Major histocompatibility complex gene (MHC) can encode antigen-presenting molecule to initiate immune response of the body, which has great potential in animal disease-resistance breeding. In this study, the correlation between MHC-DQB2 gene and Coccidia infection in Shanbei white cashmere goats (Capra hircus) was analyzed to provide basic data for the mining and application of disease-resistant genes in goats. MHC-DQB2 gene exon 2 was analyzed by bioinformatics and population genetics in 104 Shanbei white cashmere goats, infection of Coccidia was detected and the correlation between DQB2 and coccidiosis was analysed. A total of 15 alleles and 27 genotypes were obtained. The results showed that DQB2 had high polymorphism and under positive selection pressure in the course of evolution, the frequency of each allele in the population had significant difference. In this study, the infection rate of Coccidia was 71.43%, and moderate intensity infection (1×105≥oocyst per gram (OPG) ≥1×104) was predominant. Correlation analysis revealed that DQB2*03 allele and 0311 genotype were negatively correlated with Coccidia infection (P＜0.05), indicating that DQB2*03 allele and 0311 genotype might probably be the susceptibility allele or genotype of coccidiosis. The results of this study will help to clarify the role of MHC molecular markers in the immunity of Shanbei white cashmere goats, provide experimental data support for the correlation between MHC genes and diseases, and promote the application in disease-resistance breeding.
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Received: 07 January 2019
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Corresponding Authors:
ylqulei@126.com
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