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| Identification of Chromosomal Ploidy and DNA Content in Xinjiang Armeniaca vulgaris by Flow Cytometry |
| LI Wen-Wen, LIU Li-Qiang, PAMIER Ai-Ni, WANG Ya-Nan, CHENG Gong, LIAO Kang* |
| Featured Fruit Tree Research Center, Xinjiang Agricultural University, Urumqi 830052, China |
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Abstract The wild apricot (Armeniaca vulgaris) distributed in Yili Valley of Xinjiang, China, is a relic community of the Tertiary warm temperate broad-leaved forest. It has a vast distribution area, large seedling population and abundant genetic diversity. In this study, different materials (young leaves and old leaves) of wild apricot were treated by different preservation methods (fresh, refrigerated, liquid nitrogen preservation). Using Medicago sativa as external standard, the cell ploidy and DNA content of wild apricot were determined by screening and optimizing 10 common dissociation solutions through flow cytometry (FCM). The results showed that the optimum mixture of MgSO4 buffer (MgSO4) and Lysis buffer (LB01) was used to detect the fresh tender leaves, and its coefficient of variation (CV) was the lowest, and the sample rate was 150 events/μL. Aging leaves could not produce a clear main peak. The CV value of samples increased with the increase of cold storage time. The CV value of young leaves within 4 d cold storage was less than 5%. The CV value of the cell nuclear suspension prepared by the old leaves and liquid nitrogen leaves was higher, showing a peak of poor resolution and more background fragments. The results showed that 20 populations of 2 populations of A. vulgaris were diploid and the content of DNA was 288~321 Mb/C. Fresh young leaves or tender leaves in cold storage within 4 days are ideal materials for FCM. The optimized mixture of MgSO4-LB01 was the best dissociation solution for apricot. FCM could be used to detect ploidy and DNA content of apricot plants quickly and accurately. The results of this study provide technical support for ploidy breeding and omics research of apricot plants. The ploidy and DNA content of wild apricot in Xinjiang are preliminarily discussed, which provide important information for the future study of molecular genetics and cytogenetics.
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Received: 13 August 2018
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Corresponding Authors:
* liaokang01@163.com
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