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| Differences of pH Values, Nutrients and Bacterial Diversities Between Canker Diseased and Healthy Carya cathayensis Trees |
| LIN Ma-Shui1*, ZHANG Mei1*, CHAI Ying-Fei1, WANG Bing-Xuan1, WANG Shi-Ying1, FAN Yan-Di1, WU Chou-Fei2, GUO Ming1, LIN Hai-Ping1** |
1 Local and National Joint Engineering Laboratory of Biopesticide high-efficient Preparation, Zhejiang A&F University, Lin'an 311300, China;
2 College of Life Sciences, Huzhou University, Huzhou 313000, China |
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Abstract The large outbreak of canker disease has posed a serious threat to the sustainable development of hickory (Carya cathayensis) industry. For the purpose of studying the effect of bacterial diversity and function of C. cathayensis on canker disease, this research adopted 16S rRNA gene high-throughput sequencing, compared the differences of pH values, nutrients and bacterial diversity in sick parts and healthy parts, bark and xylem, respectively of healthy hickory trees and diseased trees during diseased period of canker disease, and the correlation were also analyzed. The results indicated that the pH values of the bark and xylem of healthy hickory trees respectively were 7.03 and 7.63. The neutral pH was significantly higher than that of acidic hickory trees (P<0.05). The tannin and soluble sugar contents of healthy hickory bark were 0.317% and 3.348% respectively, while that of the xylem were 0.079% and 0.626% respectively, of which all the values were remarkably higher the values of diseased trees (P<0.05). The reducing sugar contents in tree bark and xylem of healthy hickory trees were 0.865% and 0.420%, respectively, of which both were lower than that of diseased hickory trees (P<0.05). The amount of bark bacterial operational taxonomic units (OTU) in healthy hickory trees was significantly higher than that in diseased trees, while the amount of xylem bacterial OTU was significantly lower than that in diseased trees. The amount of the main bacterial genus found in healthy hickory barks, sick parts and free-diseased parts of diseased hickory barks were 7, 8, and 8 respectively, while the amount of that in xylem respectively were 8, 4, and 8. The species and abundance of the main bacterial genus in the barks and xylem of healthy and diseased hickory trees presented remarkable differences (P<0.05). The dominant bacteria genus lived in healthy hickory bark were Bdellovibrio, Acidocella, and Sphingomonas, and their abundance were 0.13%, 0.01%, and 15.32%, respectively. While the dominant bacterial genus founded in xylem were Massilia and Sphingomonas, and their abundance were 1.2% and 4.41%, respectively, of which both were remarkably higher than that of diseased hickory trees (P<0.05). In the diseased hickory trees, the dominant bacterial genus of sick and free-diseased bark both were Byssovorax and Methylobacterium. In diseased parts, the abundance of these 2 genera were 7.56% and 6.46% respectively, both higher than that in free-diseased parts. Both of the abundance of these 2 bacteria genera in 2 parts, tree bark, of the diseased C. cathayensis trees were obviously higher than that of the healthy hickory trees (P<0.05). In diseased parts xylem and free-diseased parts xylem of diseased hickory trees, Methylobacterium, Luedemannella, and Byssovorax performed as the dominant genera, and their abundance were 2.36%, 0.35%, 2.35% and 1.76%, 1.81%, and 0.55% respectively. All these figures were significantly higher than that of healthy hickory trees (P<0.05). The index of Ace, Chao, Shannon, and Simpson in the bark and xylem of healthy hickory trees were 599, 603, 6.59, 0.98 and 440, 454, 6.35, 0.95. All values were significantly higher than that of diseased hickory trees (P<0.05). The analysis of redundancy analysis (RDA) and monte carlo testing indicated that the impact of pH value, soluble sugar, and reducing sugar on the dominant bacteria genera in the bark and xylem of C. cathayensis trees were remarkable and significant (P<0.05). This research result could be considered as a reference for a sustaining and healthy operation run by the hickory forest.
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Received: 20 July 2018
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Corresponding Authors:
**, zjlxylhp@163.com
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| About author:: * The authors who contribute equally |
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