Abstract：Abstruct：Cells derived from porcin fetus expresse PDX-1, GLUT-2,PCNA, Glucagon, somatostatin，polypeptide.Nestin was weakly expressed.These cells have strong viability,It were subcloned over 58 passages during 13 months and identified as pancreatic stem cells by immunochemical method。
The cell can form three-diemention islet-like structure by induction in vitro.It stongly express insulin and ck-19 after forming ICCs.This result reveal that the cell is pancreatic stem cells and pancreatic stem cells do exist in pancreas,It maintains the steady of pancreas by neogenesis and differation.
At present,our laboratory can isolate and expand this cell steadily.This study can provide reference for isolation and expansion of pancreatic stem cells.Also,it can provide new source for diabetes therapy.
