Abstract：It has been demonstrated that plant receptor-like kinases play important roles in growth and development and environmental stress. Juxtamembrane region, the fragment between transmembrane and kinase domain in receptor-like kinases, spans about 20 amino acids in length, but the sequences are not conserved. It was demonstrated in animal receptor kinases that juxtamembrane region regulates autophosohorylation activity. Rice kinases were cloned and expressed and analyzed autophosphorylation activities in our previous study. To investigate the effect of juxtamembrane region on the autophosohorylation activity of rice receptor-like kinases, truncated kinases without juxtamembrane region were expressed, and their autophosphorylation activities were analyzed. The results showed that, the absence of juxtamembrane region did not affect the expression of kinase proteins, while 14 out of 17 truncted kinases abolished the activity as detected by autophosphorylation, indicate that the juxtamembrane region may play vital role for the regulation of plant receptor-like kinases.
李莉云 靳苗静 刘茜 陈浩 刘国振. 水稻类受体激酶的近膜区对激酶活性的影响研究[J]. , 2008, 16(2): 0-.
Li-Yun LI Miao-Jing JIN Qian LIU Hao CHEN . Effects of juxtamembrane region on the autophosohorylation activity of rice receptor-like kinases. , 2008, 16(2): 0-.
