Abstract：Abstract The effects of different microelement concentrations in the culture medium on browning and differentiation of callus derived from mature embryos of three elite indica rice cultivars, Guichao2, Teqing and 9311 were investigated through the callus induction, two-time subculture, pre-differentiation and differentiation in this study. The results suggested that in the culture medium of pre-differentiation and differentiation, callus browning was inhibited and callus greening frequency was improved when Guichao2 callus was cultured with 5×CC microelement content and 9311 callus was cultured with 3×CC microelement content, but no effect for Teqing callus when increasing the microelement content. This study provides a new insight into indica rice genetic improvement and transformation.
