Advances of the Research on Isolation and Culture for the Domestic Preantral Follicles In Vitro
Liu Ruihua1 Li Ming1,2 Jiao Lihong1 Zhen Xing2 Wang Hong1**
(1. State Key Laboratory of Reproductive Biology，Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China； 2. College of Biological Sciences, China Agricultural University, Beijing 100094, China )
Abstract： Abstract：The exact mechanisms regulating in vivo folliculogenesis in mammalians have only been partly unravelled. Some processes, such as the initiation of growth of primordial follicles are still poorly understood. This increases the difficulty to culture follicles in vitro. There are important species differences in regulation and timing of maturation, which makes it difficult to transpose techniques. Over recent decades, different animal follicle culture systems have been developed, and a better insight has been gained in the process of folliculogenesis, but the yield of good quality mature oocytes has been very limited. Until now, only within the mouse model, the birth of live young have been reported. With the exception of a unique experiment, where the starting tissue was composed of primordial follicles, all other culture systems could only sustain oocyte growth and development, when follicles were retrieved from the preantral follicle. In the domestic animal models (bovine, pig), the success of folliculogenesis in vitro has been very limited. At most the growth in vitro of preantral follicles could be achieved in the bovine and pig. The purpose of this review is to define the characteristics of preantral follicles growth and develop the isolation methods and culture system in vitro in domestic animals
刘瑞华1 李 明1,2 焦丽红1 郑 行2 王 红1* *. 家畜类腔前卵泡的体外分离方法和培养体系研究进展[J]. , 2003, 11(3): 313-317.
Liu Ruihua1 Li Ming1,2 Jiao Lihong1 Zhen Xing2 Wang Hong1**. Advances of the Research on Isolation and Culture for the Domestic Preantral Follicles In Vitro. , 2003, 11(3): 313-317.
