（1. 中国农业大学动物医学院, 北京 100094； 2. 安徽省农业委员会畜牧局，合肥 230001； 3. Avian Disease and Oncology Laboratory，United States Department of Agriculture，East Lansing,MI 48823, USA）
Expression of Mutant Tumor Suppressor Gene p53 in Egg-type Chicken Infected with Avian Leukosis Viruse Subgroup J
Xu Binrui1 Dong Weixing2 He Zhaoqing1 Yu Chunming1 Li Ning1 Feng Xiaoyu 1 L.F. Lee3 Maoxiang Li3
（1. College of Animal Medicine, China Agricultural University , Beijing 100094, China; 2. Animal Husbandry Bureau, Anhui Agricultural Committee, Hefei 230001 ,China; 3. Avian Disease and Oncology Laboratory， United States Department of Agriculture，East Lansing, MI 48823, USA）
Abstract：Abstract：Avian leukosis in egg-type chickens is infectious neoplastic disease caused by avian leukosis viruse subgroup J( ALV-J). Expression of mutant tumor suppressor gene p53 in egg-type chicken with avian leukosis subgroup J was detected to study cancericidally molecular mechanism of ALV-J. Using immunohistochemistry method of strep avidin-biotin complex (SABC), many organs of illed egg-type chickens, involved liver, kidney, ventriculus glandularis, tumor, heart, pancreas, bone marrow, spleen, oviduct, lung, duodenum, thymus and bursa of fabricius were studied. And large numbers of mutant p53 protein were expressed in liver, kidney, ventriculus glandularis, tumor, oviduct. Moderate expression were detected in heart, pancreas, bone marrow, spleen, lung, duodenum, thymus and bursa of fabricius. The result suggested that expression of tumor suppressor gene p53 was concerned with occurrence and development of avian leukosis subgroup J.
