Abstract：Partial genome of Tobacco vein distorting virus（TVDV）was amplified from total RNA extracted from tobacco plants infected with tobacco bushy top disease by RT-PCR. The amplified fragment comprises 1655bp，which contains partial RNA-dependent RNA polymerase（RdRp）gene，complete coat protein（CP）gene and complete movement protein（MP）gene. The intergenic region of RdRp gene and CP gene consists 201 nucleotides，which are identical in arrangement to the genus Polerovirus. Molecular homology trees were constructed based on the amino acid sequences of the RdRp，CP and MP，and the results showed that TVDV shares much more homologous with the specieses of the genus Polerovirus than other genuses of family Luteoviridae，which indicated that TVDV should be a definitive member of the genus Polerovirus in the family Luteoviridae.
