Abstract：The open read frame of ap36 gene was amplified from plasmid pMYE by PCR using a pair of special primers. The PCR production was cut by XbaI and SphI and sub-cloned into the pBMB982-304. Thus, the ap36 gene sequence located the downstream of slh motif of S-layer protein gene. Then the recombinant plasmid pBMB0588 was transformed into Bacillus thuringiensis plasmid–free derivative strain BMB171. The result showed the target protein was successful expressed as a SLH-Ap36 fusion protein. In order to investigate the induced ability of the recombinant strain, tomato and potato chips treated with the 24h cultured of recombinant strain were investigated to determine the induction of defense responses. The result showed the activity of peroxidase and the amount of proline of tomato leaves was increased 57.14% and 131.59% compared to control after treatment of 90 min, respectively. The activity of phenylalanine ammonia lyase was also higher than control after treatment of 4d. Furthermore, the treated potato showed high resistance to rot disease caused by Erwinia corotovora SCG1 compared to control.
