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2025年8月11日 星期一
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
脂滴包被蛋白基因(PLIN)多态性与鸡胴体及脂肪性状的相关性
周艳1,雷秋霞2,韩海霞2,李福伟1,高金波1,刘玮1,逯岩曹顶国2
Association on Single Nucleotide Polymorphism of Perilipin Gene (PLIN) with Carcass and Fatness Traits in Luqin Chicken(Gallus gallus)
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摘要 脂滴包被蛋白基因(perilipin, PLIN)对脂质代谢和脂肪沉积具有重要调控作用。为揭示PLIN基因多态性对鸡(Gallus gallus)胴体及脂肪性状的遗传效应,本研究以384只鲁禽B3系为研究材料,采用DNA测序和PCR-RFLP技术对PLIN进行多态性检测,在供试群体中检测到3个SNPs位点g.2272C>T、g.2319C>T和g.2467G>A。基于这3个SNPs位点,采用PHASE2.0软件构建了7个单倍型,其中C-C-G为最主要单倍型,频率高达71.94%;C-C-A和C-T-A单倍型频率最低,不足1%。基于7个单倍型构建了11个双倍型,H1H1频率最高,为52.60%;关联分析结果表明,双倍型对活体重、屠体重和腿肌重影响极显著(P<0.01),对腹脂重和腹脂率影响显著(P<0.05),对胸肌肌内脂肪含量有一定影响(P=0.094 9)。最小二乘分析结果表明,H1H3组合的活体重、屠体重、腿肌重、腹脂重和腹脂率最高,H4H6组次之,H6H6组合最低;H4H6组合胸肌肌内脂肪含量最高,H1H3组合最低。表明双倍型H6H6为劣势组合,在实际生产中应该去除, H4H6可以作为提高鲁禽鸡胴体性能和肉质风味的分子标记,在选育过程中应加强选择。本研究结果为PLIN基因应用于鸡分子育种提供了理论基础。
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周艳
雷秋霞
韩海霞
李福伟
高金波
刘玮
逯岩曹顶国
关键词 脂滴包被蛋白基因(PLIN)单倍型屠宰性状脂肪性状    
Abstract:Perilipin gene(PLIN) plays a crucial role during lipid metabolism and fat deposition. In order to reveal the genetic effects of polymorphisms in PLIN on carcass and fatness traits in chicken, PLIN gene polymorphisms in 384 Luqin chicken (Gallus gallus)were detected by DNA sequencing and PCR-RFLP methods. Three single nucleotide polymorphism (SNP) loci, g.2272C>T, g.2319C>T and g.2467G>A in PLIN gene were found, and their genetic effects on carcass and fatness traits were analyzed by sas6.12. Based on the 3 SNPs, 7 haplotypes were constructed by PHASE2.0. C-C-G was the major haplotype with the frequency of 71.94%, while the frequencies of C-C-A and C-T-A were lower than 1%. Eleven diplotypes were obtained from the 7 haplotypes, H1H1 was the dominant diplotype with the frequency of 52.60%. Statistical analysis showed that diplotype was extremely significantly associated with living body weight, carcass weight and leg muscle weight (P<0.01), and significantly associated with abdominal fat weight and percentage of abdominal fat (P<0.05), as well as breast intramuscular fat (P=0.094 9). The multiple-range test indicated that H1H3 chickens had the highest living body weight, carcass weight, leg muscle weight, abdominal fat weight and percentage of abdominal fat, followed by H4H6 chickens. H6H6 chickens were the lowest. The breast intramuscular fat content was the highest in H4H6 chickens and the lowest in H1H3 chickens. The results indicated that H6H6 was disadvantaged for chicken carcass and fatness traits, which should be removed during the breeding, while H4H6 was an advantaged diplotype which was suggested to be chosen to improve the carcass traits and meat quality of Luqin chickens. The research build the theoretical basis for the PLIN gene applied in poultry molecular breeding.
Key wordsGallus gallus    Perilipin gene(PLIN)    Haplotype    Carcass traits    Fatness traits
收稿日期: 2014-03-21     
通讯作者: 曹顶国   
引用本文:   
周艳1,雷秋霞2,韩海霞2,李福伟1,高金波1,刘玮1,逯岩曹顶国2. 脂滴包被蛋白基因(PLIN)多态性与鸡胴体及脂肪性状的相关性[J]. , 2014, 22(8): 1002-1008.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2014/V22/I8/1002
 
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