Abstract：By transfering N-3 fatty acid desaturase gene to pork to make N-6 polyunsaturated fatty acids into N-3 polyunsaturated fatty acids, a new way to supplement N-3 polyunsaturated fatty acid can be found. Subchronic toxicity effect of the genetically modified meat which containing N-3 fatty acid desaturase gene on pigs can be observed. One hundred and forty, 4~5 weeks old Sprague Dawley rats(Rattus norvegicus) were randomly divided into 7 groups according to the weight and gender, given a nutritionally balanced purified diet containing 3.75%，7.50% and 15.00%transgenic or conventional meat for 90 d. AIN-93 normal diet was used as an additional control group. Body weight, food consumption, haematological parameter and clinical biochemistry parameter were observed during the experiment period. At the end, the rats were sacrificed and organ weight measurement analysis and pathological examnation were performed. There were no significant differences of some indices of body weight, food utilization, hematology, clinical biochemistry, organ index and organ pathological examnation in transgenic meat group, compared to parental meat group and AIN-93 group. There was no adequate evidence to indicate the subchronic toxicity and adverse effects for transgenic meat which containing N-3 fatty acid desaturase gene on rats. This also provides reference data to assess comprehensive safety of N-3 fatty acid desaturase gene transgenic technology.
