摘要MxIrt1基因是从小金海棠（Malus xiaojinensis Cheng et Jiang）中克隆得到的Fe（II）转运蛋白基因，具有“ZIP基因家族”的保守功能域，推测该基因与苹果中铁的转运吸收有关。本研究构建MxIrt1基因的植物表达载体pCWIrt，通过农杆菌介导转化拟南芥IRT1突变体irt1-1。GUS染色以及Southern杂交检测结果显示，MxIrt1基因已经整合到拟南芥基因组中。在缺铁胁迫条件下，转基因植株提高了突变体irt1-1的耐缺铁能力，表明MxIrt1基因参与了铁的吸收。
Abstract：Fe(II) transporter gene MxIrt1 was cloned from Malus xiaojinensis Cheng et Jiang. MxIrt1 has the conserved domain of ZIP gene family. It was supposed to be involved in the iron absorption. Full length of MxIrt1 gene under the regulation of 35S promoter was introduced into Arabidopsis mutant irt1-1 by Agrobacterium tumefaciens. GUS staining and Southern analyses indicated that MxIrt1 gene has been integrated into Arabidopsis genome. The transgenic Arabidopsis plants showed increased tolerance to iron stress than the mutant.
