Abstract：To identify the aluminum stress induced genes of alfalfa, a forward subtractive cDNA library was constructed from AL-treated and non-treated seedling roots by suppression subtractive hybridization. Colony PCR suggested that the iniserts were between 250bp~1200bp and the library was suitable for the following work. 40 clones were randomly selecteed, secquenced and obtained 36 unique ESTs. BLASTX show that these ESTs including antioxidative stress, signal transduction, development and energy metaboly, liake peroxidase, calcium-dependent protein kinase, neutral/alkaline invertas, protein phosphatase regulator ect. Many ESTs related to other stresses were also found in our study and nine ESTs had no homology from NCBI.
