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2025年4月5日 星期六
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黑曲霉固体发酵产木聚糖酶的响应面优化设计及其水解特性的研究
刘明启1,关荣发2,陈文伟2
1. 浙江省杭州市下沙高教园区中国计量学院生命学院食品系
2.
Optimization of solid state fermentation conditions for xylanase production using response surface methodology and the enzymatic properties
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摘要 采用响应面法的中心组合设计对影响黑曲霉(Aspergillus niger JL15)固体发酵产木聚糖酶的条件进行了优化。以桔皮粉为基质,外加碳源、氮源,含水量以及发酵时间对木聚糖酶产量具有显著影响(P<0.05)。模拟二次多项式回归预测模型,并建立自变量与响应值的回归方程,获得各因素的最佳水平,即:甘油、硫酸铵的添加量分别为4.2%和3.1%,含水量为61%,发酵时间为73.4h,木聚糖酶活性最大预测值达922.9 U/克干发酵产物,实验验证值为917.7 U/克干发酵产物,高出基础培养基酶活3.2倍。酶学性质分析表明,黑曲霉木聚糖酶(XylA)的最适温度和最适pH分别为55°C 和 pH5.0。XylA的Km和Vmax分别为9.24 mg/ml和54.05 μmol/min/ml。Mn2+、Zn2+、Mg2+对XylA活性具有促进作用,而Fe3+、Cu2+对XylA活性具有强烈的抑制作用。HPLC分析结果表明,XylA的水解桦木木聚糖和麸皮不溶性木聚糖的产物均为木糖至木六糖,主要产物为木三糖。
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刘明启
关荣发
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关键词 木聚糖酶响应面法优化水解木三糖    
Abstract:The production of xylanase (XylA) by a newly isolated Aspergillus niger JL15 strain in solid-state fermentation (SSF) on orange peel was optimized by response surface methodology (RSM). Results revealed that four factors of concentrations of added glycerin and ammonium sulfate, moisture content, fermentation time had significant effect on the XylA production (P<0.05). The maximum xylanase activity (917.7 U/g dry fermentation product) obtained at 4.2% glycerin, 3.1% (NH4)2SO4 by employing orange peel as the solid substrate, 61% moisture content and 73.4-h fermentation, which was close to the predicted one (922.9 U/g), and was 3.2 times higher than that of the basic medium (218.5 U/g). The optimum temperature and pH for XylA activity were 55°C and pH5.0, respectively. XylA exhibited Km and Vmax values of 9.24 mg/ml and 54.05 μmol/min/ml, respectively. HPLC analysis showed that XylA liberated mainly xylotriose from birchwood xylan and wheat bran, which suggested the XylA might be an endo-acting xylanase.
Key wordsXylanase    Response surface methodology(RSM)    Optimization    Hydrolysis    Xylotriose
收稿日期: 2009-01-19     
通讯作者: 刘明启   
引用本文:   
刘明启1,关荣发2,陈文伟2. 黑曲霉固体发酵产木聚糖酶的响应面优化设计及其水解特性的研究[J]. , 2010, 18(1): 52-60.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I1/52
 
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