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基于SSR标记的粗山羊草遗传多样性分析*
王亚娟1,王长有1,刘新伦2,吉万全1
1. 西北农林科技大学
2.
Genetic Diversity of Aegliops tauschii Based on SSR Marker*
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摘要 粗山羊草是普通小麦遗传改良的重要基因资源。本研究利用普通小麦的SSR引物对78份粗山羊草DNA水平进行了遗传多样性分析。结果表明,粗山羊草D组的同源性与普通小麦3个基因组的同源性依次为D组>B组>A组。选取特异带稳定清晰的32对SSR引物对78份粗山羊草进行多样性分析,共扩增出308个等位变异位点,平均每对SSR引物扩增出8.14个等位变异位点,其中A组引物、B组引物和D组引物分别扩增出39个、51个和212个等位变异,平均每个位点等位变异分别为5.57个、6.38个和12.47个,D组引物检测到遗传变异最丰富,再次说明粗山羊草D组的同源性与普通小麦的D组同源性最高,同时,能在A、B引物中扩出特异性条带,说明粗山羊草D组与普通小麦的A、B基因组也有一定的同源性。UPGMA聚类结果表明,78份粗山羊草在遗传相似系数0.77时聚为6个主要类群,而且遗传聚类关系与材料的地理来源有一定的相关性。
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王亚娟
王长有
刘新伦
吉万全
关键词 粗山羊草遗传多样性 SSR    
Abstract:Aegliops tauschii,one of the wild relatives of Triticum, is an important genetic resource for wheat improvement . In this study, 78 accessions of Ae. tauschii were analyzed using 211 pairs of wheat SSR primers .Our data showed that D genome of Ae. tauschii had greatest genetic similarity with D genome of wheat, followed by wheat B genome, and the last A genome. Thirty-two polymorphic SSR primers were selected, and 308 alleles were detected in Ae. tauschii accessions with an average of 8.14 alleles per locus detected by a pair of polymorphic pairs. The primers of A genome detected 39 alleles with a mean of 5.57 per locus, primers of B genome 51 alleles with a mean of 6.38 per locus, and primers of D genome 39 alleles with a mean of 12.47 per locus, which indicated that primers of D genome detected great genetic diversity. The result concluded that D genome of Ae. tauschii had greatest genetic similarity with D genome of common wheat, and it also had some similarity with A and B genome of common wheat since the primers of the two genomes were polymorphic in D genome of Ae. Tauschii.. UPGM A cluster analysis showed that tested materials could be classified into six clusters with similarity coefficiency at 0.77, and relationship of clusters was associated with geographical distribution of materials. genetic polymorphism were detected by SSR primers of D genome. UPGM A cluster analysis showed that all test materials could be classified into six main groups at similarities co-efficiency in 0.77. The cluster results were associated with its geographical distribution.
Key wordsAegliops tauschii.    Genetic diversity    SSR
收稿日期: 2009-04-20     
通讯作者: 吉万全   
引用本文:   
王亚娟1,王长有1,刘新伦2,吉万全1. 基于SSR标记的粗山羊草遗传多样性分析*[J]. , 2010, 18(3): 493-500.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I3/493
 
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