Abstract：Five loose-curd-type cauliflower hybrids were applied to induce embryogenesis and plant regeneration via isolated microspore culture. The results indicated that the embryogenesis was mainly genotype-dependency. The highest embryo production was found in Chinglong 65, which produced an average yield of 15.5 embryos per flower bud. The germinated rate for microspore-derived embryos in loose curd cauliflowers was normally at 30%, meanwhile a great number of doubled haploid plants were obtained. Cold pretreatment to flower buds had a significant influence upon microspore embryogenesis, and a contradictory result was given regarding to tested genotypes. A big variation in growth stage and fertility for regenerated plants at heading stage and anthesis was observed, showing above 50% plants giving normal seed set and fertile capacity, which implies no longer doubling treatment in cauliflower microspore culture.
