Abstract：AFLP markers were used to increase density of the peach (Pruns persica (L.) Batsch) genetic map based on a F2 of 109 progenies derived from the cross of cultivars Okubo and Xingjin. Thirty and five new markers were obtained and 28 markers were added to the genetic map constructed previously. A total of 144 markers were assigned to 11 linkage groups (LOD 3.0), the total length of linkage map was 1167.6 covering 97.3% of the peach genome. The average length of 11 groups and the average interval between markers was 106.15 cM and 12.04 cM respectively. Compared with the previous linkage map, 9 new markers were assigned to group 9, but 5 markers previously located at this group were lost. At group 6, not only the order of the loci was changed, but also the average interval distance of them increased. Furthermore, locations of some markers were changed between group 3 and group 11, between group 1 and group 10. These inconsistencies were also discussed.
