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免疫捕捉PCR法检测西瓜细菌性果斑病研究
谢关林
浙江大学生物技术研究所;浙江大学植物保护系
Detection for Pathogen of Bacterial Fruit Blotch of Watermelon by Immuno-capture PCR
Guan-Lin XIE
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摘要 利用免疫吸附富集结合经典PCR技术建立了免疫捕捉PCR检测西瓜果斑病菌的检测法,并与直接PCR和生长检测法作了比较。结果表明,所测果斑病菌Acidovorax avenae subsp. citrulli都产生360bp左右的特异性片段,而10个不同属的对照菌株无特异性片段产生。免疫捕捉PCR检测果斑病菌的灵敏度为50-102cfu/ml,而直接PCR则在104cfu/ml,两者的灵敏度相差100倍左右。免疫捕捉PCR法对市售7种瓜种的检测发现,其中1种哈密瓜种子、2种甜瓜种子和2种西瓜种子检出果斑病菌,与人工气候箱内生长检测的发病结果基本吻合。显示了该法准确、灵敏、快速、低成本等优点。
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谢关林
关键词 西瓜果斑病菌检测PCR免疫捕捉PCR    
Abstract:An immuno-capture PCR (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli, a pathogen of bacterial fruit blotch of watermelon, has been established by combination of the ISE method with classical PCR and compared with the direct PCR method and growth-checking method. The results showed that all the strains of A. avenae subsp. citrulli tested produced 360bp specific fragments by the IC-PCR and direct PCR method, while others strains of 10 different genera showed negative PCR result. The minimum detection concentration for the IC-PCR and direct PCR method was about 50-102cfu/ml and 104cfu/ml, respectively. The sensitivity of the former is 100 times higher than that of later. Detection of 7 batches of different melon seeds from the markets by IC-PCR showed that 1 cantaloupe variety, 2 honey melon varieties and 2 watermelon varieties of seeds carried the pathogen which almost matched with the result of the growth-checking of the melon seeds. It indicates that the IC-PCR is accurate, sensitive, rapid and low cost.
Key wordsBacterial fruit    Detection    PCR    immuno-capture PCR
收稿日期: 2006-07-10     
通讯作者: 谢关林   
引用本文:   
谢关林 . 免疫捕捉PCR法检测西瓜细菌性果斑病研究[J]. , 2006, 14(6): 0-.
Guan-Lin XIE . Detection for Pathogen of Bacterial Fruit Blotch of Watermelon by Immuno-capture PCR. , 2006, 14(6): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2006/V14/I6/0
 
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