Abstract：Intergeneric transfer of plasmid vectors pSET152 and pHL212 from Donor E. coli ET12567/pUZ8002 and S17-1 to Streptomyces cinnamonensis were demonstrated and optimized. NsdA gene disruption structure was constructed by PCR-targeting system and then introduced into Streptomyces cinnamonensis BIB2005 through intergeneric conjugal transfer.The disruption of nsdA was confirmed by PCR assays. Compared with the start strain, the yield of monensin of the nsdA mutant BIB309 increased 270 percent in the level of flask.
郑应华 陈芬 熊伟 闵勇 范与庆 梁运祥 吕和平 刑仁昌. 肉桂地链霉菌接合转移体系的构建及nsdA基因中断对其次级代谢的影响[J]. , 2007, 15(6): 0-.
. Construction of the conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism. , 2007, 15(6): 0-.
