Abstract：In order to detect the interaction between Rice stripe virus (RSV) CP, SP and NSvc4, the HA-tagged or c-Myc-tagged CP, SP and NSvc4 of RSV were amplified by PCR, and then inserted into plant expression vector pEGAD or pKYLX71:35S2. The recombinant plasmids were transformed into Agrobacterium tumefaciens EHA105, and the EHA105 containing different recombinant was injected into Nicotiana benthamiana. The total proteins were isolated from Agrobacterium-infiltrated N. benthamiana leaves, and co-immunoprecipitated with HA or c-Myc antibody. The immunocomplexes were detected by Western blot with c-Myc or HA antibody to identify the interaction between these proteins. The results showed that RSV CP can interact with itself, but no interaction between other proteins was detected.
