摘要摘要：【目的】本文拟采用real time PCR技术对瘤胃液中三种功能菌即脂解厌氧弧杆菌（Anaerovibrio lipolytica，AL）,琥珀酸酸丝状杆菌(Fibrobacter succinogene，FS)和黄色瘤胃球菌(Ruminococcus flavefaciens，RF)的16S rDNA基因拷贝数进行测定，并建立起反映瘤胃液中三种功能菌的数量的方法。【方法】在相同日粮的情况下，不同个体牛在同一时间点、以及相同个体牛在不同时间点时采集瘤胃液微生物样品，并总DNA。根据三种菌16SrDNA保守序列设计特异性引物，利用Real time PCR技术考察三种功能菌的数量差异和变化。【结果】通过数据统计分析发现，不同个体动物间的三种功能菌的数量在相同时间点的差异不显著；而在不同时间点相同个体动物瘤胃中三种功能菌数量差异显著（P＜0.05）。
Abstract：Abstract: 【Objective】Using Real time PCR method detect three functional bacterias’ 16S rDNA copies in rumen liquid such as Anaerovibrio lipolytica, Fibrobacter succinogene, Ruminococcus flavefaciens, and building the method to reflect these bacteria quantities.【Method】At the same diet conditions ,collecting the rumen liquid microbe of different cows at the same time and the same cow at the different time. And extracting their total DNA. Design the special primers according to 16S rDNA. Then using real time PCR detect the different quantities of three functional bacteria.【Result】Three functional bacteria quantities at the same time have little different, however, there are obviously different about the same cow at different time point (P＜0.05). The main reason perhaps were the error on progress or method of collecting rumen bacteria samples.
李旦 王加启. 应用Real time PCR方法测定瘤胃液功能菌群数量[J]. , 2008, 16(5): 0-.
Dan Li . Using Real time PCR detect the functional bacteria quantities in rumen liquid. , 2008, 16(5): 0-.
