Construction of PR Domain Containing 1, with ZNF Domain(PRDM 1) Lentivirus Vector and Its Effect on Human Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Differentiation into Germ Cells
Abstract：PR domain containing 1, with ZNF domain(PRDM 1) is of great importance in the formation of primordial germ cell (PGC). A lot of reports focused on its function in the development of embryonic stem cell (ESC) to PGC, but it is unclear whether it has effects on the induction differentiation of adult stem cells or not. In this study, PRDM1 gene was cloned(GenBank No. JX154081.1) of mouse(Mus musculus), Lentivirus expression vector pCDH-PRDM1 was constructed and prepared Lentivirus particles using 293T cells, then, human umbilical cord mesenchymal stem cell (hUC-MSC) were transduced with PRDM1 gene-carrying virus. Results showed that PRDM1 could overexpress in hUC-MSC and upregulate the expression of some germ cell associated genes including stage-specific embryonic antigen-1(SSEA-1), developmental pluripotency associated 3 Dppa3(STELLA), stem cell growth factor receptor(C-KIT) and sex determining region Y-box 2(SOX2). The research provides important basis for further study of PRDM1, and is helpful to the research on induced differentiation of hUC-MSC towards germ cells.
李娜1,吴江1,华进联2. PR结构域蛋白1(PRDM1)慢病毒表达载体的构建及其对脐带间充质干细胞(hUC-MSC)向生殖细胞诱导分化的影响[J]. , 2014, 22(9): 1074-1082.
1, 1,. Construction of PR Domain Containing 1, with ZNF Domain(PRDM 1) Lentivirus Vector and Its Effect on Human Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Differentiation into Germ Cells. , 2014, 22(9): 1074-1082.
