Abstract：To clarify the relationship between brassinosteroid(BR) and Ca2+ signal transduction and their regulating mechanism in plant growth and development, 2D-DIGE technology combined with mass spectrometry analysis was used to identify proteins differentially expressed in d61-4(BR insensitive mutant in rice(Oryza sativa L. ssp. Japanica)) and brd1-3(BR synthesis mutant in rice) by comparing with the wild type plant. A protein significantly upregulated both in d61-4 and brd1-3 was identified. The protein was related to Ca2+ signal transduction network(putative calmodulin, PCAM). This gene was overexpressed in Arabidopsis thaliana. Transgenic Arabidopsis lines overexpressing PCAM were smaller in size, and plant growth period was prolonged. The expression of SaurAC(a key gene in BR signal transduction pathway) in transgenic strains was obviously down-regulated. These results suggest a role of PCAM in the signal transduction of BR is as a negative regulator of the plant growth and development.
黄新 王凤茹 董金皋. PCAM蛋白功能分析及其与油菜素内酯(BR)信号转导关系[J]. , 2012, 20(12): 1407-1413.
wang fengru. Function Analysis of Putative Calmodulin(PCAM) and the Relationship between PCAM and Brassinosteroid(BR) Signal Transductionin. , 2012, 20(12): 1407-1413.
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