Breeding of New Wheat (Triticum aestivum) Cultivar Using Gene Pyramiding Method for High-quality and Stripe Rust Resistance
HE Rui1, MA Rui1, GUO Ying1, DU Jiu-Yuan1, ZHANG Li-Jun1, ZHAN Zong-Bing1, WANG Hong-Mei2, LIU Feng3, BAI Bin1,*
1 Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 Institute of Biotechnology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 Scientific Research Management Office, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
Abstract：Improvement of disease resistance, quality, and high yield simultaneously has been a long-standing goal in wheat (Triticum aestivum) breeding. Breeding new high-yielding dryland wheat varieties with both stripe rust resistance and high quality is particularly important for developing modern cold-arid agriculture in Gansu province. In this study, 'Ta04-44-1-3' wheat line developed from recurrent selection population selection of Taigu genic male-sterile wheat carrying the known high-quality glutenin subunit genes Ax1/Ax2* and Bx7+By8, as well as the pleiotropic stripe rust resistance genes Lr34/Yr18/Pm38/Sr57 and Lr27/Yr30/Sr2/Sb3 was utilized as the female parent, and 'Lantian 19', which possessed the known high-quality glutenin subunits Bx7+By8, Bx7+By9, Glu-A3d, as the male parent for hybridization. Using specific molecular markers for the high-quality glutenin subunit and stripe rust resistance genes, combined with disease resistance evaluation, quality assessment, and field-based comprehensive trait selection, a new winter wheat variety named 'Lantian 214' was developed. This variety combines high-quality, stripe rust resistance, stress tolerance, and high yield, making it suitable for dryland conditions. The variety possessed the high-quality glutenin subunits Ax1/Ax2*, Bx7+By8, Bx7+By9, Glu-A3d, as well as the pleiotropic resistance genes Lr34/Yr18/Pm38/Sr57 and Lr27/Yr30/Sr2/Sb3. Disease resistance evaluation revealed that the variety was moderately susceptible (MS) to mixed races of stripe rust at the seedling stage but exhibited immunity to prevalent mixed races at the adult plant stage. Grain quality analysis indicated that its grain test weight was 829 g/L, protein content was 13.4%, wet gluten content was 33.2%, dough stability time was 6.8 min, water absorption rate was 58.8%, maximum resistance to extension was 426 E.U, and extensibility area was 92 cm2, meeting the standards for medium-strong gluten wheat. The development of new winter wheat cultivar 'Lantian 214' provides a high-quality, disease-resistant winter wheat variety suitable for dryland production in the mountainous southeastern Gansu. It also offers new parental germplasm for future efforts in pyramiding multiple genes for breeding in this region.
何瑞, 马瑞, 郭莹, 杜久元, 张礼军, 展宗冰, 王红梅, 刘风, 白斌. 基因聚合培育兼具优质和抗条锈病小麦新品种[J]. 农业生物技术学报, 2026, 34(5): 1048-1058.
HE Rui, MA Rui, GUO Ying, DU Jiu-Yuan, ZHANG Li-Jun, ZHAN Zong-Bing, WANG Hong-Mei, LIU Feng, BAI Bin. Breeding of New Wheat (Triticum aestivum) Cultivar Using Gene Pyramiding Method for High-quality and Stripe Rust Resistance. 农业生物技术学报, 2026, 34(5): 1048-1058.
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