Abstract：The adrenoceptor beta 3 (ADRB3) gene encodes a G protein-coupled membrane surface receptor with multiple roles in regulating lipolysis and energy expenditure. The aim of this study was to investigate the single nucleotide polymorphisms (SNP) in the untranslated region (UTR) of the ADRB3 gene by Sanger sequencing of PCR products and MassARRAY, and analyze their association with reproductive traits in Hu sheep (Ovis aries). The results showed that 4 SNP loci were identified in the UTR of the ADRB3 gene: g.42G>T, g.2377G>T, g.2501T>A and g.2622G>A. Each of these loci exhibits 3 genotypes and the dominant genotypes were all TT except for g.2622G>A whose dominant genotype was GA. All SNPs showed middle polymorphism (0.25<PIC<0.5) except g.42G>T (PIC<0.25). χ2 tests indicated that the genotype distributions of all SNPs were in Hardy-Weinberg equilibrium (P>0.05). Association analysis revealed that for the g.42 G>T locus, the litter size in parity 1 was significantly higher in individuals with the GG genotype than those with the TT genotype, which was significantly higher than the GT genotype (P<0.05). The litter size in parity 2 was significantly higher in individuals with the GG genotype than those with the GT and TT genotypes. The lambing interval of parity 2~3 in individuals with the GT and TT genotypes was significantly shorter than those with the GG genotype (P<0.05). For the g.2377G>T locus, the lambing interval of parity 2~3 in individuals with the GT genotype was significantly shorter than those with the TT genotype (P<0.05). At the g.2501T>A locus, the litter size in parity 4 was significantly higher in individuals with the TT and TA genotype than those with the AA genotype (P<0.05). For the g.2622G>A locus, the litter size in parity 3 was significantly higher in individuals with the GA genotype than those with the AA genotype (P<0.05). Diplotype analysis showed that 5 haplotypes and 15 diplotypes were detected at 4 SNPs loci in the experimental population. Association analysis of 9 (frequency>0.05) haplotypes with reproductive traits revealed that the individuals with the H5H5 (TTTTTTGG) diplotypes had higher litter size. In conclusion, the ADRB3 gene can be used as a candidate gene for molecular markers of reproductive traits. This study provides a reference for accelerating the breeding process of high fertility Hu sheep.
朱臻, 孟旭铭, 李晓彤, 刘彤, 寇志远, 仓振, 茆达干. 湖羊β3肾上腺素能受体基因多态性及其与繁殖性状的关联分析[J]. 农业生物技术学报, 2025, 33(10): 2164-2173.
ZHU Zhen, MENG Xu-Ming, LI Xiao-Tong, LIU Tong, KOU Zhi-Yuan, CANG Zhen, MAO Da-Gan. Polymorphism of ADRB3 Gene and Its Association Analysis with Reproductive Traits in Hu Sheep (Ovis aries). 农业生物技术学报, 2025, 33(10): 2164-2173.
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