Genome-wide Identification of ZF-HD Family and Its Expression Pattern Analysis in Magnolia sinostellata
ZHU Sen-Yu1, CHEN Xiao-Jun1, MA Jing-Jing1, REN Ming-Jie1, WANG Jing-Jing1, WU Chao1, TIAN Feng1, LIU Zhi-Gao1, FAN Min-Liang2,*, SHEN Ya-Mei1,*
1 Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants/School of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, China; 2 Zhejiang Academy of Forestry, Hangzhou 310023, China
Abstract：Stress from canopy shade is currently endangering a very small population of the indigenous Magnolia sinostellata found in Zhejiang province. A group of transcription factors known as zinc finger homeodomain (ZF-HD) is crucial for the plant development as well as the response to biotic and abiotic stress. In order to gain a better understanding of the mechanism underlying the MsZF-HD gene family under low-light stress, this study aimed to identify the members of the ZF-HD gene family and investigate their expression patterns based on transcriptome data. Twelve members of the MsZF-HD family were found using transcriptome data from M. sinostellata leaves and genomic data from M. biondii. The 12 MsZF-HDs located in the cell nucleus, and MsZF-HD2b also located in chloroplast. The ZF-HD_dimer structural domain was present in all members of the MsZF-HD gene family, which was revealed by phylogenetic analysis to be separated into 4 subfamilies. Additionally, the number and types of motifs were substantially conserved among the members of the same subfamily. According to GO analysis, members of the MsZF-HD family played a significant role in the expansion and evolution of M. sinostellata. Tissue-specific expression study revealed that most MsZF-HDs genes were substantially expressed in M. sinostellata leaf buds and leaves. MsZF-HD8 was highly expressed in both stems and leaves, while MsZF-HD13 and MsZF-HD14 were highly expressed in both flowers and stems, indicating that they might be involved in stem elongation and the regulation of floral development. Additionally, it was discovered that MsZF-HDs all responded favorably to low-light stress after studying the expression pattern of the MsZF-HDs gene under shade stress. Under low-light stress, the expression of MsZF-HD1a, MsZF-HD2b, MsZF-HD1b, MsZF-HD1c, MsZF-HD2a and MsZF-HD14 decreased. MsZF-HD13 gene expression was significantly upregulated after 21 and 28 d of low-light treatment (P<0.05). MsZF-HD11, MsZF-HD12 and MsZF-HD8 showed significant upregulation of gene expression after 7 d of low-light treatment (P<0.05), followed by a downward trend. The ZF-HD family members of M. sinostellata were identified in this study and it was discovered that there was a significant expression response to low-light stress in leaf, stem and other tissues. MsZF-HD13 and MsZF-HD8 were found to be key genes in the response to shading to coordinate reproductive growth, stem elongation and leaf senescence. This study offers a theoretical framework for examining M. sinostellata's shade response mechanism.
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